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A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
A Book

by Jianghui Hou

  • Publisher : Academic Press
  • Release : 2020-04-24
  • Pages : 420
  • ISBN : 0128226439
  • Language : En, Es, Fr & De
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A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

A Laboratory Guide to Human Physiology

A Laboratory Guide to Human Physiology
Concepts and Clinical Applications

by Stuart Ira Fox

  • Publisher : Unknown Publisher
  • Release : 1984
  • Pages : 323
  • ISBN : 9780697047243
  • Language : En, Es, Fr & De
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Oral Histology Laboratory Guide

Oral Histology Laboratory Guide
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 1978
  • Pages : 73
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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A Laboratory Guide to the Mammalian Embryo

A Laboratory Guide to the Mammalian Embryo
A Book

by David K. Gardner,Michelle Lane,Andrew J. Watson

  • Publisher : Oxford University Press
  • Release : 2004-01-08
  • Pages : 394
  • ISBN : 9780195142266
  • Language : En, Es, Fr & De
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Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

The Paracellular Channel

The Paracellular Channel
Biology, Physiology, and Disease

by Jianghui Hou

  • Publisher : Academic Press
  • Release : 2018-06-08
  • Pages : 248
  • ISBN : 0128146362
  • Language : En, Es, Fr & De
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The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

NIH Guide for Grants and Contracts

NIH Guide for Grants and Contracts
A Book

by National Institutes of Health (U.S.)

  • Publisher : Unknown Publisher
  • Release : 1979
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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The Laboratory Guide

The Laboratory Guide
A Manual of Practical Chemistry for Colleges and Schools Specially Arranged for Agricultural Students

by Sir Arthur Herbert CHURCH

  • Publisher : Unknown Publisher
  • Release : 1894
  • Pages : 292
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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The laboratory guide for students of agricultural chemistry

The laboratory guide for students of agricultural chemistry
A Book

by sir Arthur Herbert Church

  • Publisher : Unknown Publisher
  • Release : 1882
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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NIH Guide for Grants and Contracts

NIH Guide for Grants and Contracts
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 1979
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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American Journal of Physiology

American Journal of Physiology
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 1992
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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"The William Townsend Porter memorial volume": v. 158.

Skin Barrier Function

Skin Barrier Function
A Book

by T. Agner

  • Publisher : Karger Medical and Scientific Publishers
  • Release : 2016-02-04
  • Pages : 164
  • ISBN : 3318055867
  • Language : En, Es, Fr & De
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Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.

The Impact of Food Bioactives on Health

The Impact of Food Bioactives on Health
in vitro and ex vivo models

by Kitty Verhoeckx,Paul Cotter,Iván López-Expósito,Charlotte Kleiveland,Tor Lea,Alan Mackie,Teresa Requena,Dominika Swiatecka,Harry Wichers

  • Publisher : Springer
  • Release : 2015-04-29
  • Pages : 338
  • ISBN : 3319161040
  • Language : En, Es, Fr & De
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“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.

Alternatives to Laboratory Animals

Alternatives to Laboratory Animals
ATLA.

by Anonim

  • Publisher : Unknown Publisher
  • Release : 1990
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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A Laboratory Guide in Pharmacology

A Laboratory Guide in Pharmacology
A Book

by Torald Hermann Sollmann

  • Publisher : Unknown Publisher
  • Release : 1917
  • Pages : 345
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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A Laboratory Manual and Study Guide for Anatomy and Physiology

A Laboratory Manual and Study Guide for Anatomy and Physiology
A Book

by Kenneth G. Neal,Barbara H. Kalbus

  • Publisher : Unknown Publisher
  • Release : 1983
  • Pages : 406
  • ISBN : 9780808714491
  • Language : En, Es, Fr & De
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Laboratory Guide in Pharmacology

Laboratory Guide in Pharmacology
A Book

by Harald Groth Oxholm Holck,Tom Saburo Miya

  • Publisher : Unknown Publisher
  • Release : 1968
  • Pages : 163
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Laboratory Guide in Pharmacology

Laboratory Guide in Pharmacology
A Book

by Tom S. Miya,Harald Groth Oxholm Holck

  • Publisher : Burgess International Group Incorporated
  • Release : 1973
  • Pages : 136
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Laboratory Animal Science

Laboratory Animal Science
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 1999
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Laboratory guide to human physiology

Laboratory guide to human physiology
concepts and clinical applications

by Stuart Ira Fox

  • Publisher : Unknown Publisher
  • Release : 1976
  • Pages : 323
  • ISBN : 9780697045447
  • Language : En, Es, Fr & De
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Claudins

Claudins
Methods and Protocols

by Kursad Turksen

  • Publisher : Humana Press
  • Release : 2011-07-02
  • Pages : 447
  • ISBN : 9781617791840
  • Language : En, Es, Fr & De
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Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.