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Analytical Techniques in Biosciences

Analytical Techniques in Biosciences
From Basics to Applications

by Stanley C. Udedi,Jonathan C. Ifemeje,Chukwuebuka Egbuna,Muhammad Ajmal Shah,Kingsley C. Patrick-Iwuanyanwu,Azhar Rasul

  • Publisher : Academic Press
  • Release : 2021-06-01
  • Pages : 500
  • ISBN : 0128227990
  • Language : En, Es, Fr & De
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Analytical Techniques in Biosciences: From basics to applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in research laboratories across the biosciences. The chapters are grouped into two parts. Part I presents chapters that deals with the basic introductory protocols and sample preparatory guidelines for an error free analysis. This section also contains chapters that detail the various analytical techniques such as spectroscopy and mass spectrometry techniques, chromatographic techniques, electrophoretic techniques and molecular weight determination techniques. Part two presents chapters that capture various applied techniques in molecular biology. Techniques for nucleic acid assessments, polymerase chain reaction, DNA sequencing, enzyme application in genetic manipulation, and those used in immunology and Immunochemistry are presented as well. This book draws from experts from key institutions around the globe, who have simplified the chapters in a way that it will be useful to early-stage researchers as well as advanced scientists. The book is also carefully structured and integrated sequentially to aid flow, consistency and continuity. This is a must-have reference for graduate students and researchers in the biosciences. Presents basic analytical protocols, sample preparatory guidelines and conventional techniques in biosciences. Details the various biochemical and molecular biology techniques including spectroscopic techniques, chromatographical techniques, centrifugation techniques and titrimetric techniques. Discuses recent advances on novel techniques for screening, identification and characterization of novel molecules. Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, application of biosensors in biomedical research and automated methods of analysis and robotics.

Bioanalytics

Bioanalytics
Analytical Methods and Concepts in Biochemistry and Molecular Biology

by Friedrich Lottspeich,Joachim Engels

  • Publisher : John Wiley & Sons
  • Release : 2018-03-26
  • Pages : 1134
  • ISBN : 3527339191
  • Language : En, Es, Fr & De
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Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a birdï¿1⁄2s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
A Book

by Rajan Katoch

  • Publisher : Springer Science & Business Media
  • Release : 2011-07-19
  • Pages : 441
  • ISBN : 9781441997852
  • Language : En, Es, Fr & De
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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Analytical Biotechnology

Analytical Biotechnology
A Book

by Thomas G.M. Schalkhammer

  • Publisher : Birkhäuser
  • Release : 2012-11-28
  • Pages : 331
  • ISBN : 3034881010
  • Language : En, Es, Fr & De
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Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Statistical Methods in Biology

Statistical Methods in Biology
Design and Analysis of Experiments and Regression

by S.J. Welham,S.A. Gezan,S.J. Clark,A. Mead

  • Publisher : CRC Press
  • Release : 2014-08-22
  • Pages : 608
  • ISBN : 1439808783
  • Language : En, Es, Fr & De
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Written in simple language with relevant examples, Statistical Methods in Biology: Design and Analysis of Experiments and Regression is a practical and illustrative guide to the design of experiments and data analysis in the biological and agricultural sciences. The book presents statistical ideas in the context of biological and agricultural sciences to which they are being applied, drawing on relevant examples from the authors’ experience. Taking a practical and intuitive approach, the book only uses mathematical formulae to formalize the methods where necessary and appropriate. The text features extended discussions of examples that include real data sets arising from research. The authors analyze data in detail to illustrate the use of basic formulae for simple examples while using the GenStat® statistical package for more complex examples. Each chapter offers instructions on how to obtain the example analyses in GenStat and R. By the time you reach the end of the book (and online material) you will have gained: A clear appreciation of the importance of a statistical approach to the design of your experiments, A sound understanding of the statistical methods used to analyse data obtained from designed experiments and of the regression approaches used to construct simple models to describe the observed response as a function of explanatory variables, Sufficient knowledge of how to use one or more statistical packages to analyse data using the approaches described, and most importantly, An appreciation of how to interpret the results of these statistical analyses in the context of the biological or agricultural science within which you are working. The book concludes with a guide to practical design and data analysis. It gives you the understanding to better interact with consultant statisticians and to identify statistical approaches to add value to your scientific research.

Ana Techniqs in Biotechnology

Ana Techniqs in Biotechnology
A Book

by Goutam Bhowmik

  • Publisher : Tata McGraw-Hill Education
  • Release : 2011
  • Pages : 244
  • ISBN : 9780070700130
  • Language : En, Es, Fr & De
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'Analytical Techniques in Biotechnology' by Bhowmik and Bose offers a unique compendium of fundamental experiments, which forms the crucial foundation to understand this contemporary subject that has enormous impact on many other branches of life sciences. In addition to its simple and lucid language, the main focus of the book is to equip a beginner with the skill and ability required to conduct independent experimentation and research in laboratories. Carefully structured to cover the complete spectra of subjects under the umbrella of 'Biotechnology', this book is sure to prove a ready reference for students, faculties and researchers, all alike!

Analytical Techniques in Biotechnology

Analytical Techniques in Biotechnology
A Book

by Suzy Hill

  • Publisher : Unknown Publisher
  • Release : 2016-05-26
  • Pages : 264
  • ISBN : 9781682862384
  • Language : En, Es, Fr & De
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Biotechnology is a rapidly progressing field of science and technology. It integrates principles and applications from diverse fields such as genomics, immunology, and many more. This book strives to equip the reader with thorough knowledge about various techniques of applied biotechnology, its applications in the areas of medicine, agriculture, environment, etc., along with details about the latest equipment and analytical tools which would help the reader gain an in-depth perspective about the subject. This book elucidates new techniques and their applications in a multidisciplinary approach and is apt for students of biotechnology and associated disciplines of study.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
A Book

by Keith Wilson,John Walker

  • Publisher : Cambridge University Press
  • Release : 2010-03-04
  • Pages : 744
  • ISBN : 0521516358
  • Language : En, Es, Fr & De
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Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications
A Book

by Neetu Mishra,Anupam Jyoti

  • Publisher : Bentham Science Publishers
  • Release : 2019-10-30
  • Pages : 153
  • ISBN : 9811422850
  • Language : En, Es, Fr & De
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Advances in biomedical research have had a profound effect on human health outcomes over the last century. Biophysical, biochemical and cellular techniques are now the backbone of modern biomedical research. Understanding these laboratory techniques is a prerequisite for investigating the processes responsible for human diseases and discovering new treatment methods. Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications Provides information about basic and advanced analytical techniques applied in specific areas of life science and biomedical Key Features: - Book chapters present a broad overview of sophisticated analytical techniques used in biophysics, biochemistry and cell biology. - Techniques covered include in vitro cell culture techniques, flow cytometry, real time PCR, X-ray crystallography, RNA sequencing - Information about industrial and biomedical applications of techniques, (drug screening, disease models, functional assays, disease diagnosis, gene expression analysis and protein structure determination) is included. The book is an excellent introduction for students (as a textbook) and researchers (as a reference work). The information it presents will prepare readers to understand and develop research methods in life science laboratories for different projects and activities.

Instrumental Analysis in the Biological Sciences

Instrumental Analysis in the Biological Sciences
A Book

by M. H. Gordon

  • Publisher : Springer Science & Business Media
  • Release : 2012-12-06
  • Pages : 244
  • ISBN : 1468415212
  • Language : En, Es, Fr & De
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Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

Surface Analysis and Techniques in Biology

Surface Analysis and Techniques in Biology
A Book

by Vincent S. Smentkowski

  • Publisher : Springer Science & Business Media
  • Release : 2014-03-18
  • Pages : 326
  • ISBN : 3319013602
  • Language : En, Es, Fr & De
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This book summarizes the main surface analysis techniques that are being used to study biological specimens/systems. The compilation of chapters in this book highlight the benefits that surface analysis provides. The outer layer of bulk solid or liquid samples is referred to as the surface of the sample/material. At the surface, the composition, microstructure, phase, chemical bonding, electronic states, and/or texture is often different than that of the bulk material. The outer surface is where many material interactions/reactions take place. This is especially true biomaterials which may be fabricated into bio-devices and in turn implanted into tissues and organs. Surfaces of biomaterials (synthetic or modified natural materials) are of critical importance since the surface is typically the only part of the biomaterial/bio-device that comes in contact with the biological system. Analytical techniques are required to characterize the surface of biomaterials and quantify their impact in real-world biological systems. Surface analysis of biological materials started in the 1960’s and the number of researchers working in this area have increased very rapidly since then, a number of advances have been made to standard surface analytical instrumentation, and a number of new instruments have been introduced.

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
A Book

by Andreas Hofmann,Samuel Clokie

  • Publisher : Cambridge University Press
  • Release : 2018-03-31
  • Pages : 962
  • ISBN : 1107162270
  • Language : En, Es, Fr & De
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A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Data Analysis for the Life Sciences with R

Data Analysis for the Life Sciences with R
A Book

by Rafael A. Irizarry,Michael I. Love

  • Publisher : CRC Press
  • Release : 2016-10-04
  • Pages : 376
  • ISBN : 1498775861
  • Language : En, Es, Fr & De
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This book covers several of the statistical concepts and data analytic skills needed to succeed in data-driven life science research. The authors proceed from relatively basic concepts related to computed p-values to advanced topics related to analyzing highthroughput data. They include the R code that performs this analysis and connect the lines of code to the statistical and mathematical concepts explained.

Instrumental Analysis in the Biological Sciences

Instrumental Analysis in the Biological Sciences
A Book

by M. H. Gordon

  • Publisher : Springer Science & Business Media
  • Release : 2012-12-06
  • Pages : 244
  • ISBN : 1468415212
  • Language : En, Es, Fr & De
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Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

Advanced Chromatographic and Electromigration Methods in BioSciences

Advanced Chromatographic and Electromigration Methods in BioSciences
A Book

by I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl

  • Publisher : Elsevier
  • Release : 1998-09-28
  • Pages : 1125
  • ISBN : 9780080858685
  • Language : En, Es, Fr & De
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This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted. Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Nuclear Analytical Methods in the Life Sciences 1994

Nuclear Analytical Methods in the Life Sciences 1994
A Book

by Jan Kucera,Ivan Obrusník,Enrico Sabbioni

  • Publisher : Springer Science & Business Media
  • Release : 2013-06-29
  • Pages : 754
  • ISBN : 1475760256
  • Language : En, Es, Fr & De
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Nuclear Analytical Methods in the Life Sciences •1994 is a forefront survey of key presentations from the 1993 International Conference on Nuclear Analytical Methods in the Life Sciences. Sponsored by the International Atomic Energy Agency (IAEA), this useful volume covers the spectrum of multidisciplinary research on both the methodological aspects and the development of nuclear analytical methods and their applications in the life sciences. The book is divided into six sections covering related material. These sections are: Methodology of Nuclear Analytical Methods; Environmental Applications; Biomedical Applications; Analysis of Biological Samples; Quality Assurance and Comparison with Other Methods; and a section dealing with miscellaneous issues, such as programs offered by the IAEA.

Advances in Biological Science Research

Advances in Biological Science Research
A Practical Approach

by Surya Nandan Meena,Milind Naik

  • Publisher : Academic Press
  • Release : 2019-05-17
  • Pages : 580
  • ISBN : 0128174986
  • Language : En, Es, Fr & De
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Advances in Biological Science Research: A Practical Approach provides discussions on diverse research topics and methods in the biological sciences in a single platform. This book provides the latest technologies, advanced methods, and untapped research areas involved in diverse fields of biological science research such as bioinformatics, proteomics, microbiology, medicinal chemistry, and marine science. Each chapter is written by renowned researchers in their respective fields of biosciences and includes future advancements in life science research. Discusses various research topics and methods in the biological sciences in a single platform Comprises the latest updates in advanced research techniques, protocols, and methods in biological sciences Incorporates the fundamentals, advanced instruments, and applications of life science experiments Offers troubleshooting for many common problems faced while performing research experiments

Quantitative Investigations in the Biosciences using MINITAB

Quantitative Investigations in the Biosciences using MINITAB
A Book

by John Eddison

  • Publisher : CRC Press
  • Release : 1999-12-20
  • Pages : 472
  • ISBN : 9781584880332
  • Language : En, Es, Fr & De
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Until recently, acquiring a background in the basic methodological principles that apply to most types of investigations meant struggling to obtain results through laborious calculations. The advent of statistical software packages has removed much of the tedium and many of the errors of manual calculations and allowed a marked increase in the depth and sophistication of analyses. Although most statistics classes now incorporate some instruction in using a statistics package, most introductory texts do not. Quantitative Investigations in the Biosciences using MINITAB fills this void by providing an introduction to investigative methods that, in addition to outlining statistical principles and describing methods of calculations, also presents essential commands and interprets output from the statistics package MINITAB. The author introduces the three basic elements of investigations-design, analysis, and reporting-using an extremely accessible approach that keeps mathematical detail to a minimum. He groups statistical tests according to the type of problem they are used to examine, such as comparisons, sequential relationships, and associations. Quantitative Investigations in the Biosciences using MINITAB draws techniques and examples from a variety of subjects, ranging from physiology and biochemistry through to ecology, behavioral sciences, medicine, agriculture and horticulture, and complements the mathematical results with formal conclusions for all of the worked examples. It thus provides an ideal handbook for anyone in virtually any field who wants to apply statistical techniques to their investigations.

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology
A Book

by S.L. Salzberg,D.B. Searls,S. Kasif

  • Publisher : Elsevier
  • Release : 1998-06-19
  • Pages : 368
  • ISBN : 9780080860930
  • Language : En, Es, Fr & De
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Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Basic Methods in Molecular Biology

Basic Methods in Molecular Biology
A Book

by Leonard Davis

  • Publisher : Elsevier
  • Release : 2012-12-02
  • Pages : 399
  • ISBN : 044460149X
  • Language : En, Es, Fr & De
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Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.