Download Biotin and Other Interferences in Immunoassays Ebook PDF

Biotin and Other Interferences in Immunoassays: A Concise Guide is aimed at clinical laboratory scientists, medical technologists and pathologists who are often the first individuals contacted by a clinician when a laboratory test result does not correlate with clinical presentation. Research scientists working in diagnostics companies will also find this information essential. Sources of errors in non-immunoassay based methods used in clinical chemistry and toxicology laboratory are also discussed so readers can get all important information from one concise guide. This succinct, user-friendly reference provides the necessary information to address high levels of biotin in clinical laboratory results. Discusses issues of biotin interferences and ways to avoid them for accurate clinical laboratory results Provides sources of errors in non-immunoassay based methods used in clinical chemistry and toxicology laboratories Highlights how to handle specimens in the lab and how to eliminate the effect of biotin in precious samples

Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Presents up-to-date information Provides extensive cross-referencing Covers theory and practice in full detail Written by leading authorities

Accurate Results in the Clinical Laboratory: A Guide to Error Detection and Correction, Second Edition, provides a comprehensive review of the factors leading to errors in all areas of clinical laboratory testing. This trusted guide addresses interference issues in all laboratory tests, including patient epigenetics, processes of specimen collection, enzymes and biomarkers. Clinicians and laboratory scientists will both benefit from this reference that applies discussions to both accurate specimen analysis and optimal patient care. Hence, this is the perfect reference for clinical laboratorians, from trainees, to experienced pathologists and directors. Provides comprehensive coverage across endocrine, oncology, hematology, immunohistochemistry, immunology, serology, microbiology, and molecular testing Includes new case studies that highlight clinical relevance and errors to avoid Highlights the best titles published within a variety of medical specialties Reviewed by medical librarians and content specialists, with key selections compiled in their annual list

This practical, easy-to-use guide, named to Doody’s Core Titles 2013, addresses interference issues in all laboratory tests, including patient epigenetics, process of specimen collection, enzymes, biomarkers. Clinicians and laboratory scientists can therefore rely on one reference which speaks to both their needs of accurate specimen analysis and optimal patient care. Erroneous hospital and pathology laboratory results can be confusing and problematic, especially in acute care situations. While some factors creating interference, can be identified in the laboratory, detecting many others is often dependent on clinical details unavailable to the laboratory scientists or pathologists. Therefore, clinicians must become proficient in identifying such erroneous reports, and working with pathologists and laboratory scientists so that they can understand the source of such interferences, correct the results, and then decide what course of action must be followed for proper patient management. Named to Doody’s Core Titles 2013, a collection development tool for health sciences libraries of all sizes, by Doody Enterprises Practical information for both clinicians and laboratory scientists, presented in the form of tables and charts for easy reference Focus on range and sources of interferences rather than details of toxicologic mechanisms which are well covered in toxicology textbooks Covers interferences across endocrine, oncology, hematology, immunohistochemistry, immunology, serology, microbiology, and molecular testing

The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing. www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com

Thyroid and Parathyroid Disorders in Children: A Practical Handbook offers a focused and practical approach to diagnostic and therapeutic (both medical and surgical) interventions in management of thyroid and parathyroid diseases in children. It is divided into two sections: thyroid and parathyroid, with each section discussing the use of the laboratory testing and radiographic modalities in the study of these glands. This guides the reader to know the utility and limitations of the findings when approaching a child with these conditions. Written by international experts in the fields of pediatric endocrinology and endocrine surgery, it is ideal for trainees, practicing physicians and professionals who care for children with endocrine conditions. Key Features Discusses differences in recognition and management of thyroid and parathyroid disorders in children compared with adults, with both common and rare disorders described along with their medical and surgical treatment techniques. Aids the practicing physicians, endocrinologists, trainees, and fellows in understanding the complex concepts in a practical way. Fills the gap in the market for a much-needed book concentrated on endocrine neck diseases of childhood.

The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and blotting technology), and diagnostics (immunoassay, histopathology, and gene probes). Recently, usage of the system has been extended to include other areas. This volume covers these new applications and methodologies including hybridoma technology, bioaffinity sensors, affinity targeting, and drug delivery, as well as cross-linking, immobilization, and fusogenic studies.

Case Presentations in Chemical Pathology is a compilation of medical records with clinical history, diagnosis, and recommended treatment. It discusses the symptoms and causes of the disease being investigated. It provides sample of laboratory results that is correlated to a possible malady. Some of the topics covered in the book are the ethanol-related liver problem; definition of hyperprolactinaemia; explanation of abnormal plasma magnesium presence in the blood; enzyme defect in acute intermittent porphyria; causes of elevated ketones in a patient’s blood and urine; and description of androgen insensitivity syndrome. The classification and clinical diagnoses of hypercalcaemia are fully covered. The definition and symptoms of Conn’s syndrome are discussed in detail. The text describes in depth the causes of euthyroid hyperthyroxinaemia. The causes of hypermagnesaemia are completely presented. A chapter is devoted to the origins of respiratory alkalosis and hyponatraemia. The book can provide useful information to doctors, students, and researchers.

Wound Healing, Tissue Repair and Regeneration in Diabetes explores a wide range of topics related to wound healing, tissue repair and regeneration, putting a special focus on diabetes and obesity. The book addresses the molecular and cellular pathways involved in the process of wound repair and regeneration. Other sections explore a wide spectrum of nutritional supplements and novel therapeutic approaches, provide a comprehensive overview, present various types of clinical aspects related to diabetic wounds, including infection, neuropathy, and vasculopathy, provide an exhaustive review of various foods, minerals, supplements and phytochemicals that have been proven beneficial, and assess future directions. This book is sure to be a welcome resource for nutritionists, practitioners, surgeons, nurses, wound researchers and other health professionals. Explains diabetic wounds and their complications Assesses the role of nutraceuticals, herbal supplements and other modalities for use in treating diabetic wounds Provides protocols for diabetic wound management

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

Rapid tests, also known as point-of-care tests, have been in use for decades in the clinical and medical area and have become increasingly popular as an efficient screening method for conducting on-site analysis thanks to their simplicity, speed, specificity and sensitivity. Nowadays, rapid tests are widely applied for clinical, drug, food, forensic and environmental analysis and fields of application are rapidly increasing together with advances in the technology. The growing interest in rapid tests and their expanding application in diverse fields, together with requirements of improved sensitivity, reliability, multiple detection capacity and robustness, are prompting innovation in the design of novel platforms, and in the exploitation of innovative detection strategies. The book covers advances in materials, technology and test design.

The poster abstracts accepted for the 71st AACC Annual Scientific Meeting & Clinical Lab Expo. AACC is a global scientific and medical professional organization dedicated to clinical laboratory science and its application to healthcare. Our leadership in education, advocacy and collaboration helps lab professionals adapt to change and do what they do best: provide vital insight and guidance so patients get the care they need.

Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.

Present Knowledge in Nutrition: Basic Nutrition and Metabolism, Eleventh Edition, provides an accessible, referenced source on the most current information in the broad field of nutrition. Now broken into two volumes and updated to reflect scientific advancements since the publication of the last edition, the book includes expanded coverage on basic nutrition, metabolism and clinical and applied topics. This volume provides coverage of macronutrients, vitamins, minerals and other dietary components and concludes with new approaches in nutrition science that apply to many, if not all, of the nutrients and dietary components presented throughout the reference. Advanced undergraduate, graduate and postgraduate students in nutrition, public health, medicine and related fields will find this resource useful. In addition, professionals in academia and medicine, including clinicians, dietitians, physicians, health professionals, academics and industrial and government researchers will find the content extremely useful. The book was produced in cooperation with the International Life Sciences Institute (https://ilsi.org/). Provides an accessible source of the most current, reliable and comprehensive information in the broad field of nutrition Features new chapters on topics of emerging importance, including the microbiome, eating disorders, nutrition in extreme environments, and the role of nutrition and cognition in mental status Covers topics of clinical relevance, including the role of nutrition in cancer support, ICU nutrition, supporting patients with burns, and wasting, deconditioning and hypermetabolic conditions

Nowadays, the implementation of novel technological platforms in biosensor-based developments is primarily directed to the miniaturization of analytical systems and lowering the limits of detection. Rapid scientific and technological progress enables the application of biosensors for the online detection of minute concentrations of different chemical compounds in a wide selection of matrixes and monitoring extremely low levels of biomarkers even in living organisms and individual cells. This book, including 16 chapters, characterizes the present state of the art and prospective options for micro and nanoscale activities in biosensors construction and applications.

Comprised of illustrative clinical cases, this unique pocket guide presents descriptions of patients who have symptoms, physical signs or laboratory abnormalities that they believe are due to disorders of the endocrine system (hormone secreting glands and overall metabolism) but which are not, or probably are not, due to an endocrine disorder. These are common situations in the clinical practice of endocrinology. Each chapter includes clinical cases illustrating differing presentations and outcomes, and each individual case description is followed by a discussion that includes the differential diagnosis of these symptoms, signs and/or lab abnormalities and why they are not likely due to endocrine disease or, alternatively, why and how a deeper exploration for endocrine disorders might be needed. In all cases, an emphasis is placed on listening to the patient and providing a respectful and compassionate response and approach to evaluation and management of the proposed disorder. Discussions are referenced whenever reference material is available, and evidence-based clinical practice guidelines are presented whenever applicable. Topics discussed include chronic and adrenal fatigue, obesity, anxiety and depression, sweating and flushing, alcohol- and opioid-induced symptoms, low testosterone, pseudo-hypoglycemia and pseudo-Cushing's syndrome, among others. Clinical endocrinologists, primary care physicians and related allied medical professionals will find Management of Patients with Pseudo-Endocrine Disorders a valuable resource in their clinical practice with these common but often challenging patients.

A user-friendly, pocket-sized reference for all physicians faced with endocrine care and challenges in hospitalized patients, this handbook covers the most common issues leading to an inpatient endocrine consult, providing differential diagnoses, a reasonable and practical approach to investigating and managing the condition, and advice for follow-up. Conditions discussed include thyrotoxicosis and thyroid storm, calcium disorders, osteoporosis, Cushing's syndrome, pheochromocytoma and paraganglioma, primary hyperaldosteronism, hypoglycemia in diabetic and non-diabetic patients, and endocrine issues during pregnancy, among others. Suggestions for further reading are included, providing more context for well-established clinical approaches. Written by experts with years of experience providing endocrinology consultations in a hospital setting, Handbook of Inpatient Endocrinology is a valuable, high-yield resource for endocrine residents and fellows, but it will be equally useful for any busy hospitalist or primary care physician when endocrinology consults are not available.

Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.

Escherichia coli is a versatile organism and very diverse. Members of this species vary from very pathogenic agents causing different types of diseases including meningitis, gastroenteritis, and septicemia, just to cite a few, to harmless organisms living in the intestines of both humans and animals. E. coli has also been used as a model organism for most bacteria except a few. For this reason, its study provides a huge advantage and can help understand the mechanisms involved in different processes such as pathogenesis, environmental disinfection, nutrient utilization, antibiotic resistance, and diagnostic/detection methods, and these are indeed the topics discussed in this book. The book has been divided into four main sections representing the different facets of E. coli applications, which include disease, biotechnology, environmental engineering and innovative approaches to detection, and lastly its physiology and cell biology. Such processes can be applied to the study of other organisms as well considering the development of diversity; for example, many organisms are capable of horizontal gene transfer, which is capable of increasing the fitness of the bacterial organisms involved and has a great impact on the control of such bacterial organism.

Taking an interdisciplinary approach that emphasizes the adaptability of immunochemical and related bioanalytical methods to a variety of matrices, Immunoassay and Other Bioanalytical Techniques describes the strength and the versatility of these methods in a wide range of environmental and biological measurement applications. With contribut