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Epigenetics Methods

Epigenetics Methods
A Book

by Trygve Tollefsbol

  • Publisher : Academic Press
  • Release : 2020-07-08
  • Pages : 736
  • ISBN : 0128194154
  • Language : En, Es, Fr & De
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In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods Written at verbal and technical levels that can be understood by scientists and students alike Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics

Plant Epigenetics: Methods and Protocols

Plant Epigenetics: Methods and Protocols
A Book

by Igor Kovalchuk

  • Publisher : Methods in Molecular Biology
  • Release : 2018-11-05
  • Pages : 264
  • ISBN : 9781493979561
  • Language : En, Es, Fr & De
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This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Population Epigenetics

Population Epigenetics
Methods and Protocols

by Paul Haggarty,Kristina Harrison

  • Publisher : Humana Press
  • Release : 2017-03-17
  • Pages : 206
  • ISBN : 9781493969012
  • Language : En, Es, Fr & De
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This detailed volume focuses on population epigenetics, which is of increasing interest to policy makers searching for explanations for complex epidemiological observations and conceptual models on which to base interventions. With a concentration on DNA methylation, the emphasis is on the most commonly studied epigenetic phenomenon that is most amenable to further study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. /divAuthoritative and practical, Population Epigenetics: Methods and Protocols aims to aid population researchers in exploiting the latest insights into epigenetics to improve our understanding of the factors that influence human health and longevity.

Cancer Epigenetics

Cancer Epigenetics
Methods and Protocols

by Ramona G. Dumitrescu,Mukesh Verma

  • Publisher : Humana Press
  • Release : 2012-02-24
  • Pages : 492
  • ISBN : 9781617796111
  • Language : En, Es, Fr & De
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The epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation. More recently, epigenetic changes have been observed in early stages of tumor development and together with the genetic alterations have been defined as abnormalities, necessary for cancer initiation and progression. In, Cancer Epigenetics: Methods and Protocols, expert researchers reviewed these epigenetics changes in different tumor types and described several technologies that are currently available to detect epigenetic changes. These technologies have lead to a better understanding of the processes in normal and cancerous cells. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Cancer Epigenetics: Methods and Protocols aids scientists in continuing to study epigenetic alterations used in clinical practice as biomarkers of early cancerous lesions or markers of progression and prognosis.

Epigenetics Protocols

Epigenetics Protocols
A Book

by Trygve O. Tollefsbol

  • Publisher : Springer Science & Business Media
  • Release : 2004
  • Pages : 302
  • ISBN : 1592598285
  • Language : En, Es, Fr & De
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Recent advances in epigenetic research as well as the development of exciting new technologies have helped greatly in unraveling the many mysteries of nontraditional genetic processes. In Epigenetics Protocols, hands-on researchers describe state-of-the-art methods for epigenetic analysis, including recent breakthrough techniques that have great potential in the rapidly expanding field of non-Mendelian genetics. The authors provide techniques for the analysis of chromatin remodeling, such as histone acetylation and methylation. In addition, methods in newly developed and especially promising areas of epigenetics, such as telomere position effects, quantitative epigenetics, and ADP ribosylation are covered. There is also an updated analysis of techniques involving DNA methylation and its role in the modification, as well as the maintenance, of chromatin structure. Of special interest are potentially revolutionary techniques. These include methods for determining changes in native chromatin, methods of microarray analysis applied to epigenetics, and methylation-sensitive single-strand conformation techniques. The methods are suitable not only for studying fundamental biological processes, but also for investigating possible therapeutic interventions and such diseases as cancer. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Epigenetics Protocols offers investigators readily reproducible techniques that will further promote progress in this critically important field.

Plant Epigenetics and Epigenomics

Plant Epigenetics and Epigenomics
Methods and Protocols

by Charles Spillane,Peter McKeown

  • Publisher : Humana
  • Release : 2021-03-09
  • Pages : 272
  • ISBN : 9781071601815
  • Language : En, Es, Fr & De
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This second edition volume expands on the previous edition with a look at the latest techniques in plant epigenetics and epigenomic research. Chapters in this book cover topics such as whole genome methylome analysis; analysis of DNA methylation; chromatin analysis of metabolic gene clusters in plants; plant epigenetic stress memory induced by drought; small non-coding RNA analysis in seeds of forest tree species; and a discussion on epigenetics inheritance in plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Epigenetics and Epigenomics: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers, and aims to advance this field of study by paving the way for integrating epigenetic mechanisms into models of plant function during development and evolution.

Epigenetics of Exercise and Sports

Epigenetics of Exercise and Sports
Concepts, Methods, and Current Research

by Stuart M Raleigh

  • Publisher : Academic Press
  • Release : 2021-01-15
  • Pages : 400
  • ISBN : 9780128206829
  • Language : En, Es, Fr & De
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Exercise and Sports Epigenetics: Concepts, Methods, and Current Research explains fundamental epigenetic processes and how these are altered by exercise and sports. After a brief review of fundamental epigenetic biology, this all-new volume in the Translational Epigenetics series offers step-by-step instruction in how epigenetic factors are investigated for their influence over exercise related traits of human physiology, disease, and injury. The current state of knowledge in the field and recent findings are discussed in-depth, illuminating how exercise and sports performance may epigenetically modify our physiology, disease and injury risks, and how this knowledge can be applied in personalized exercise approaches, diagnostics, and treatment. This book also explores the shortcomings of explaining exercise related phenomena using only genomics and traditional biochemical techniques, setting the scene for a paradigm shift in exercise biology. In addition, over a dozen international specialists contribute chapters on exercise and sports epigenetics, and their influence over metabolism, obesity, aging, immunity, and neurological disease, as well as the epigenetic impacts of concussions and sports doping. A concluding chapter discusses ongoing themes in the field and outlooks for future research. Thoroughly examines fundamental concepts in exercise and sports epigenetics, methods for new research, and known impacts for human physiology, disease, and clinical outcomes Discusses exercise and sports epigenetics in relation to metabolism, obesity, aging, immunity, and neurological disease, concussion, and sports doping, among other topics Features chapter contributions from international experts in the field

Epigenetic Epidemiology

Epigenetic Epidemiology
A Book

by Karin B. Michels

  • Publisher : Springer Science & Business Media
  • Release : 2012-01-03
  • Pages : 448
  • ISBN : 9400724942
  • Language : En, Es, Fr & De
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The exploding field of epigenetics is challenging the dogma of traditional Mendelian inheritance. Epigenetics plays an important role in shaping who we are and contributes to our prospects of health and disease. While early epigenetic research focused on plant and animal models and in vitro experiments, population-based epidemiologic studies increasingly incorporate epigenetic components. The relevance of epigenetic marks, such as DNA methylation, genomic imprinting, and histone modification for disease causation has yet to be fully explored. This book covers the basic concepts of epigenetic epidemiology, discusses challenges in study design, analysis, and interpretation, epigenetic laboratory techniques, the influence of of age and environmental factors on shaping the epigenome, the role of epigenetics in the developmental origins hypothesis, and provides the state of the art on the epigenetic epidemiology of various health conditions including childhood syndromes, cancer, infectious diseases, inflammation and rheumatoid arthritis, asthma, autism and other neurodevelopmental disorders, psychiatric disorders, diabetes, obesity and metabolic disorders, and atherosclerosis. With contributions from: Peter Jones, Jean-Pierre Issa, Gavin Kelsey, Robert Waterland, and many other experts in epigenetics!

Computational Epigenetics

Computational Epigenetics
Bioinformatic Methods for Epigenome Prediction, DNA Methylation Mapping and Cancer Epigenetics

by Christoph Bock

  • Publisher : Unknown Publisher
  • Release : 2008
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Epigenetics of Cancer Prevention

Epigenetics of Cancer Prevention
A Book

by Anupam Bishayee,Deepak Bhatia

  • Publisher : Academic Press
  • Release : 2018-11-27
  • Pages : 442
  • ISBN : 0128125667
  • Language : En, Es, Fr & De
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Epigenetics of Cancer Prevention, Volume Ten is the first to look at epigenetics and chemoprevention together. Although there is numerous scientific data available on how epigenetics can lead to cancer and how chemoprevention can be beneficial in the treatment of, or improvement of quality of life, together they will set an advanced understanding for the reader in this upcoming field of chemoprevention influencing epigenetics. This book discusses molecular epigenetic targets of natural products, such as green tea polyphenols, curcumin and resveratrol, and organ specific epigenetic targets related to diverse types of cancer, for example prostate, colorectal, breast, lung and skin cancers. Additionally, it encompasses a discussion on research methods and limitations to study epigenetics and epigenomics of chemopreventive drugs and personalized cancer treatment with phytochemicals. The book is ideal for cancer researchers, health care professionals and all individuals who are interested in cancer prevention research and its clinical applications, especially in natural remedies. Lists natural agents, including nutraceuticals, and their effects on normal or tumor genome Addresses various epigenetic systems and mechanisms in the regulation and support of the mammalian genome Discusses how various parts of dietary phytochemicals can influence or modify epigenetic mechanisms in several types of cancer

Developmental Human Behavioral Epigenetics

Developmental Human Behavioral Epigenetics
Principles, Methods, Evidence, and Future Directions

by Livio Provenzi,Rosario Montirosso

  • Publisher : Academic Press
  • Release : 2020-11-20
  • Pages : 188
  • ISBN : 0128192631
  • Language : En, Es, Fr & De
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Developmental Human Behavioral Epigenetics: Principles, Methods, Evidence, and Future Directions, Volume 23, a new volume in the Translational Epigenetics series, offers the first systematic account of theoretical G79 frameworks, methodological approaches, findings, and future directions in the field of human behavioral epigenetics. Featuring contributions from leading scientists and international researchers, this book provides a comprehensive overview of human behavioral epigenetics, with a close examination of evidence gathered to-date from animal models, challenges of human-based research and clinical translation, pathways towards drug discovery, and next steps in research. Areas of focus include prenatal stress exposures, preterm behavioral epigenetics, intergenerational exposures, trauma and neglect, socio-economic conditions, maternal caregiving and attachment, study design, and epigenetics and psychotherapy. Enables more effective study design and methods application in behavioral epigenetics research across human and animal models Examines findings in human behavioral epigenetics to-date Features contributions from leading international researchers in behavioral epigenetics

Cancer Epigenetics for Precision Medicine

Cancer Epigenetics for Precision Medicine
Methods and Protocols

by Ramona G. Dumitrescu,Mukesh Verma

  • Publisher : Humana
  • Release : 2019-09-17
  • Pages : 298
  • ISBN : 9781493993833
  • Language : En, Es, Fr & De
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This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Cancer Epigenetics for Precision Medicine: Methods and Protocols is a valuable resource to help researchers and scientists identify these specific biomarkers and work towards the prevention, diagnosis, and prognosis of different cancers in the future.

Structural and Epigenetic Analysis of the Human Genome

Structural and Epigenetic Analysis of the Human Genome
A Book

by Gene E. Ananiev

  • Publisher : Unknown Publisher
  • Release : 2008
  • Pages : 165
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Practical Epigenetics, Best Practices in Modern Belief-Change-Methods

Practical Epigenetics, Best Practices in Modern Belief-Change-Methods
For Holistic and Energy Medicine Practitioners

by Bruce Dickson,Professor of Politcal Science Bruce Dickson

  • Publisher : Createspace Independent Publishing Platform
  • Release : 2016-03-08
  • Pages : 144
  • ISBN : 9781530421381
  • Language : En, Es, Fr & De
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Volume 6 in New Directions in Holistic Brain Balance "Epigenetics" is Bruce Lipton's word for DNA changes caused by our beliefs-not our genes. His idea of malleable genes is spreading, penetrating more and more deeply into popular imagination. Over our lifetime, as we change our beliefs, our habits change, even the lines on our palms and feet change. Our face changes. Why not also our DNA? When it's possible, permitted and clear, disturbed mental-emotional baggage can be released from our unconscious, all the way down to the level of our genes. This allows our genes-DNA to heal as well. Given the innovations since the 1950s in belief change work, a summary of Best Practices in Modern Belief Change Work, is timely. Bruce Lipton updated DNA-change theory. What about methods? Bruce endorses Psych-K's use of muscle testing for uncovering and addressing faulty beliefs as effective. I hope every Reader will try Psych-K personally. However, wonderful and effective as Psych-K is, professional grade methods offer much more. Belief Change Work has been popular in the US since Albert Ellis in the 1950s. Since then, scores of Belief Change methods have been innovated: - Cognitive Behavioral Therapy, - Touch for Health muscle-testing, - affirmations, - NLP, - meridian tapping, - DNA-change-work and - ecumenical spirituality. These are compatible and can easily be used together for more effective Belief Change Work. The Hero's Journey of Self healing is a path of exploring out-dated beliefs and habits, upgrading them to more workable ones, aligned with our highest good and brightest future. Let's pack for our Hero's Journey of Self-healing. We'll need curiosity and courage to uncover the origins of beliefs no longer working for us. Along the way we'll uncover and learn from our earlier choices. We'll clear the recording media of our psyche, erase old programs and re-record it with new habits, beliefs, behaviors, more to our liking. Self-healers are like Theseus in the Labyrinth hunting for the Minotaur. You decide to find the Minotaur in your own internal labyrinth when you uncover and realize the havoc it's wreaking inside. The Hero/Heroine decides it's up to you to go down there, root him out and transform him. Wait, before you jump, you need a method to deal with the Minotaur once you find him. Theseus carried a sword with him. What's your method of choice? In 2016 we have many more Tools That Heal. We don't use a sword any more and we don't use violence for Belief Change Work. This book is one person's method. It's repeated in four versions, each one more detailed. If you get stuck, find a Healing Buddy or a safe paid coach you can trust and learn from. Table of Contents, abbreviated 1 - Perceived inability to evolve; Power of beliefs to keep us stuck, a story 2 - Why change my beliefs? 3 - Best practices from 65 years of conventional Belief Change Work 4 - List of known professional-grade Belief Change modalities 5 - What is a belief? What's a value? An attitude? 6 - We have beliefs on multiple levels 7 - Recent hopeful developments 8 - Multiple Intelligences 2.0 9 - How deep is my issue? 10 - Feeling Real changes we wish to make 11 - Stuck? Try "chunking down" 12 - Want to change a belief? Talk to your Habit Librarian 13 - One person's Belief-Change Protocol, 2016 14 - Top 20 Most popular self-defeating beliefs Health Intuitive Bruce Dickson offers initial gift session by phone-Skype. All books: http: //www.amazon.com/Bruce-Dickson-MSS/e/B007SNVG46 Health Intuitive Bruce Dickson offers initial gift phone-Skype sessions between 8 AM and 9:00 PM PST. Phone 626-627-0296 Skype: SelfHealingCoach

Epigenetic Drug Discovery

Epigenetic Drug Discovery
A Book

by Wolfgang Sippl,Manfred Jung

  • Publisher : John Wiley & Sons
  • Release : 2019-03-25
  • Pages : 504
  • ISBN : 3527343148
  • Language : En, Es, Fr & De
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This broad view of epigenetic approaches in drug discovery combines methods and strategies with individual targets, including new and largely unexplored ones such as sirtuins and methyl-lysine reader proteins. Presented in three parts - Introduction to Epigenetics, General Aspects and Methodologies, and Epigenetic Target Classes - it covers everything any drug researcher would need in order to know about targeting epigenetic mechanisms of disease. Epigenetic Drug Discovery is an important resource for medicinal chemists, pharmaceutical researchers, biochemists, molecular biologists, and molecular geneticists.

Epigenetic Regulation and Epigenomics

Epigenetic Regulation and Epigenomics
A Book

by Robert A. Meyers

  • Publisher : John Wiley & Sons
  • Release : 2012-10-02
  • Pages : 1254
  • ISBN : 3527668624
  • Language : En, Es, Fr & De
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Epigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The "epigenome" is a parallel to the word "genome," and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA and the chromatin proteins that associate with it. Methylation, for example, can silence a nearby gene and seems to be involved in some cancers. Epigenetics is beginning to form and take shape as a new scientific discipline, which will have a major impact on Medicine and essentially all fields of biology. Increasingly, researchers are unearthing links between epigenetics and a number of diseases. Although in recent years cancer has been the main focus of epigenetics, recent data suggests that epigenetic plays a critical role in psychology and psychopathology. It is being realized that normal behaviors such as maternal care and pathologies such as Schizophrenia and Alzheimer's might have an epigenetic basis. It is also becoming clear that nutrition and life experiences have epigenetic consequences. Discover more online content in the Encyclopedia of Molecular Cell Biology and Molecular Medicine.

Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases
A Book

by Barbara Stefanska,David J. MacEwan

  • Publisher : Humana Press
  • Release : 2016-12-14
  • Pages : 197
  • ISBN : 9781493967414
  • Language : En, Es, Fr & De
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This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicology series, the book includes the kind of detail and implementation advice to encourage success in the lab. Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics.

Cancer Epidemiology

Cancer Epidemiology
A Book

by Mukesh Verma

  • Publisher : Unknown Publisher
  • Release : 2009
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Epigenetic Gene Expression and Regulation

Epigenetic Gene Expression and Regulation
A Book

by Suming Huang,Michael D Litt,C. Ann Blakey

  • Publisher : Academic Press
  • Release : 2015-10-19
  • Pages : 482
  • ISBN : 0128004711
  • Language : En, Es, Fr & De
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Epigenetic Gene Expression and Regulation reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies. The book shows how these heritable mechanisms allow individual cells to establish stable and unique patterns of gene expression that can be passed through cell divisions without DNA mutations, thereby establishing how different heritable patterns of gene regulation control cell differentiation and organogenesis, resulting in a distinct human organism with a variety of differing cellular functions and tissues. The work begins with basic biology, encompasses methods, cellular and tissue organization, topical issues in epigenetic evolution and environmental epigenesis, and lastly clinical disease discovery and treatment. Each highly illustrated chapter is organized to briefly summarize current research, provide appropriate pedagogical guidance, pertinent methods, relevant model organisms, and clinical examples. Reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies Helps readers understand how epigenetic marks are targeted, and to what extent transgenerational epigenetic changes are instilled and possibly passed onto offspring Chapters are replete with clinical examples to empower the basic biology with translational significance Offers more than 100 illustrations to distill key concepts and decipher complex science

GAW20

GAW20
Methods and Strategies for the New Frontiers of Epigenetics and Pharmacogenomics

by Nathan L. Tintle,Justo Lorenzo Bermejo

  • Publisher : Unknown Publisher
  • Release : 2018
  • Pages : 329
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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