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Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass
A Book

by Arindam Kuila,Vinay Sharma

  • Publisher : Elsevier
  • Release : 2020-02-19
  • Pages : 254
  • ISBN : 0128179546
  • Language : En, Es, Fr & De
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Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production

Improving Metabolic Engineering and Characterization of Clostridium Thermocellum for Improved Cellulosic Ethanol Production
A Book

by Beth Alexandra Papanek

  • Publisher : Unknown Publisher
  • Release : 2016
  • Pages : 91
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives for the production of biofuels is equally important. Most commonly, biofuels are produced using a crop such as corn or soybeans to feed sugars to the yeast, Saccharomyces cerevisiae for the fermentation of ethanol. Lignocellulosic biofuel production would eliminate the need for food crops and transition to biomass such as switchgrass, poplar, or corn stover. Currently, lignocellulosic biofuel production is limited primarily because of the cost of converting the biomass to fermentable sugars than can then be metabolized by yeast. To overcome this barrier, a process must be employed that can convert lignocellulosic biomass directly to fuels and chemicals quickly and affordably. Clostridium thermocellum is one of the most promising candidates for the production of advanced biofuels because of its potential ability to convert cellulose directly to ethanol without the expensive addition of enzymes. Challenges to implementing C. thermocellum on an industrial scale still exist including side product formation, slow growth, limited titers, inhibition on high solids loadings, and a limited ability to perform genetic engineering. This thesis considers all of these concerns with C. thermocellum and attempts to systematically improve each characteristic to produce an industrially relevant strain of C. thermocellum for advanced biofuel production. Metabolic engineering is applied for the elimination of undesirable fermentation products. Laboratory evolution and medium supplementation are used to improve and understand the mechanisms that influence growth rate, and systematic approaches are used to improve transformation for more efficient genetic engineering of C. thermocellum in the future.

Production of Top 12 Biochemicals Selected by USDOE from Renewable Resources

Production of Top 12 Biochemicals Selected by USDOE from Renewable Resources
Status and Innovation

by Anuj K. Chandel,Fernando Segato

  • Publisher : Elsevier
  • Release : 2021-11-01
  • Pages : 474
  • ISBN : 012823654X
  • Language : En, Es, Fr & De
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Production of Top 12 Biochemicals Selected by USDOE from Renewable Resources: Status and Innovation covers all important technological aspects of the production of biochemicals from renewable feedstock. All the important technological aspects of biomass conversion for example biomass pretreatment, enzymatic hydrolysis for cellulosic sugars production followed by the fermentation into chemicals and downstream recovery of the products is reviewed. Recent technological advancements in suitable microorganism development, bioprocess engineering for biomass conversion for cellulosic sugars production and various fermentation strategies and downstream recovery of these top 12 products is presented. Each bio-chemical selected by US Department of Energy i.e. ethanol, xylitol/sorbitol, furans (5-HMF, 2,5-FDCA,), glycerol & its derivatives, hydrocarbons) isoprene, iso-butadienes and others), lactic acid, succinic acid, 3-hydroxy propionic acid, levulinic acid and biohydrogen/biogas is included in a single book chapter. In addition to the technical aspects of these 12 biochemicals, general technological challenges dealing with lignocellulose refining, perspectives and solutions are elaborated in the book. Also, life cycle analysis, techno-economic viability, and sustainability index of biofuels/biochemicals are comprehensively reviewed in the book. covers uniquely designed scientific and technical literature on USDOE top listed biochemicals production with clear images and tables in the context of biomass valorisation Includes the clear and simplistic illustration of technological updates on biomass processing, system biology, microbial fermentation, catalysis, regeneration and monitoring of renewable energy and chemicals production Presents fast and reliable source of information on techno-economic analysis, life cycle analysis, technological scouting at industrial scale Entails fundamental aspects, recent developments in production of renewable chemicals as building block materials for commodity chemicals production

Current Status and Future Scope of Microbial Cellulases

Current Status and Future Scope of Microbial Cellulases
A Book

by Deepak K. Tuli,Arindam Kuila

  • Publisher : Elsevier
  • Release : 2021-03-26
  • Pages : 414
  • ISBN : 0128218967
  • Language : En, Es, Fr & De
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Current Status and Future Scope of Microbial Cellulases not only explores the present and future of cellulase production, it also compares solid state fermentation (SSF) and submerged fermentation (SMF) for cellulase production. Chapters explore bioprocess engineering, metabolic engineering and genetic engineering approaches for enhanced cellulase production, including the application of cellulase for biofuel production. This important resource presents current technical status and the future direction of advances in cellulase production, including application of cellulases in different sectors. Covers the present industrial scenarios and future prospect of cellulase production Describes the molecular structure of cellulase Explores genetic engineering, metabolic engineering and other approaches for improved cellulase production Includes different applications of cellulases, including their application in the bioenergy sector

Use of Process Design and Metabolic Engineering to Enhance Bioconversion of Lignocellulosic Biomass and Glycerol to Biofuels

Use of Process Design and Metabolic Engineering to Enhance Bioconversion of Lignocellulosic Biomass and Glycerol to Biofuels
A Book

by Chidozie Victor Agu

  • Publisher : Unknown Publisher
  • Release : 2016
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Recent efforts to reduce dependency on food-based substrates for industrial applications aim towards the use of inexpensive and readily available non-food based substrates such as lignocellulosic biomass (LB) and biodiesel-derived glycerol. Interestingly, the utilization of lignocellulosic sugars for biofuel production is contingent on the disruption of recalcitrant LB cell wall structure prior to enzyme hydrolysis. Disruption and hydrolysis processes generate lignocellulose-derived microbial inhibitory compounds (LDMIC) including acids, aldehydes and phenolics. Additionally, fermentation of glycerol to butanol, a next-generation biofuel, is hampered by the inability of Clostridium beijerinckii NCIMB 8052, a butanol fermentation workhorse, to efficiently metabolize glycerol. Therefore, this study investigated novel strategies for enhancing butanol and ethanol production through process design and metabolic engineering. Towards process design, the bacterium Cupriavidus basilensis ATCC®BAA-699 was used to detoxify 98% of the LDMIC present in acid-pretreated Miscanthus giganteus (MG) lignocellulosic biomass hydrolysates. Fermentation of the detoxified MG hydrolysates by C. beijerinckii resulted in 70%, 50%, and 73% improvement in acetone-butanol-ethanol (ABE) concentration, yield and productivity, respectively, when compared to the fermentation of undetoxified MG hydrolysates. The second objective was to explore metabolic engineering strategies to enhance glycerol utilization by C. beijerinckii and improve butanol production in the presence of LDMIC. To realize this objective, genes that encode glycerol dehydrogenases (Gldh) and dihydroxyacetone kinase (Dhak) in a hyper-glycerol utilizing bacterium (Clostridium pasteurianum ATCC 6013) were systematically cloned into C. beijerinckii. By over-expressing two C. pasteurianum Gldh genes (dhaD1+gldA1) as a fusion protein in C. beijerinckii, we achieved 50% increase in cell growth, ABE production (up to 40%), and enhanced rate of furfural detoxification (up to 68%) during the fermentation of furfural-challenged (4 to 6 g/L) glucose+glycerol medium. Further, co-expression of dhaD1+gldA1 resulted in significant payoff in cell growth (57%), glycerol consumption (14%), and ABE productivity (27.3%) compared to over-expression of a single Gldh. In parallel, while co-expression of dhak and gldA1 in C. beijerinckii improved glycerol consumption by 37% relative to the plasmid control, over-expression of all three genes (dhaD1+gldA1+dhak) improved butanol production by >50% in the presence of 5 and 6 g/L furfural relative to the plasmid control. Objective 3 aimed to develop a high-throughput alcohol dehydrogenase (ADH)-dependent assay for screening hyper- or hypo- butanol producing C. beijerinckii mutant libraries. Screening of the activities of ADHs from different microorganisms showed that Thermotoga hypogea derived ADH has ~7-fold activity towards butanol than ethanol. It was rationalized that T. hypogea ADH can be used to selectively quantify butanol in the presence ofethanol (e.g., in ABE broth). Objective 4 aimed to use allopurinol to inhibit xanthine dehydrogenase/oxidase and improve ethanol fermentation of LB hydrolysates by Saccharomyces cerevisiae. Allopurinol increased S. cerevisiae growth (19%), ethanol titer (21%), ethanol productivity (20%), ethanol yield (24%), and the chronological lifespan of S. cerevisiae (>16 h) during the fermentation of 100% corn stover hydrolysate. Taken together, this study encompasses novel strategies to enhance LB and glycerol utilization and potentially improve the economics of biobutanol and bioethanol production.

Biofuels Production

Biofuels Production
A Book

by Vikash Babu,Ashish Thapliyal,Girijesh Kumar Patel

  • Publisher : John Wiley & Sons
  • Release : 2013-09-09
  • Pages : 392
  • ISBN : 1118835735
  • Language : En, Es, Fr & De
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The search for alternative sources of energy to offset diminishing resources of easy and cost-effective fossil fuels has become a global initiative, and fuel generated from biomass is a leading competitor in this arena. Large-scale introduction of biofuels into the energy mix could contribute to environmentally and economicaly sustainable development on a global scale. The processes and methodologies presented in this volume will offer a cutting-edge and comprehensive approach to the production of biofuels, for engineers, researchers, and students.

Biorefineries: A Step Towards Renewable and Clean Energy

Biorefineries: A Step Towards Renewable and Clean Energy
A Book

by Pradeep Verma

  • Publisher : Springer Nature
  • Release : 2021-01-04
  • Pages : 620
  • ISBN : 9811595933
  • Language : En, Es, Fr & De
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This book provides a comprehensive account of past, present and future of the biomass based biorefineries. It is an all-inclusive and insightful compilation of recent advancements in the technology and methods used for conversion of biomass to bioenergy and other useful biochemicals. The book also focuses on the limitations of existing technologies and provides the future prospects, as well as discusses socio-economic impact of biomass based biorefineries. This book assists researchers in the area of lignocellulosic biorefineries and can be used by the students, scientist and academician as an advanced reference textbook.

Engineering of Microorganisms for the Production of Chemicals and Biofuels from Renewable Resources

Engineering of Microorganisms for the Production of Chemicals and Biofuels from Renewable Resources
A Book

by Guillermo Gosset

  • Publisher : Springer
  • Release : 2017-01-26
  • Pages : 200
  • ISBN : 3319517295
  • Language : En, Es, Fr & De
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This book reviews state of the art regarding strategies for generating and improving microbial strains designed for utilizing renewable raw materials. It discusses methods for genetically engineering of thermophilic bacteria, Saccharomyces cerevisiae, Escherichia coli and Zymomonas mobilis, as well as approaches for obtaining useful products from these renewable raw materials based on biotechnological processes using microbes to chemically transform them. However, the efficient transformation of lignocellulosic biomass or glycerol to useful products represents a major challenge: Biomass has to be treated physically and chemically to release a mixture of sugars that potentially can be employed by the microbial production strains. These hydrolytic treatments result in diverse toxic compounds being generated and released, that negatively impact strain performance. Furthermore, most of the commonly used industrial microbes do not have the natural capacity to efficiently utilize and transform the generated sugar mixtures or glycerol. The microbial species reviewed in this book possess particular advantages as production strains and are currently employed for the synthesis of numerous biofuels and chemicals. The book reviews the general and strain-specific genetic engineering strategies for the improvement of sugar mixtures and glycerol catabolism. The issue of lignocellulosic hydrolysate toxicity is addressed in several chapters, where genetic engineering and adaptive laboratory evolution strategies are reviewed and discussed. The objective of this book is to provide the current knowledge regarding strategies for the generation and improvement of microbial strains designed for the transformation of renewable raw materials into useful products. This book aims to become a reference for researchers and students working in this field.

Valorization of Biomass to Value-Added Commodities

Valorization of Biomass to Value-Added Commodities
Current Trends, Challenges, and Future Prospects

by Michael O. Daramola,Augustine O. Ayeni

  • Publisher : Springer Nature
  • Release : 2020-04-21
  • Pages : 595
  • ISBN : 3030380327
  • Language : En, Es, Fr & De
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This book presents the most up-to-date technologies for the transformation of biomass into valuable fuels, chemicals, materials, and products. It provides comprehensive coverage of the characterization and fractionation of various types of biomass and details the many challenges that are currently encountered during this process. Divided into two sections, this book discusses timely topics such as the characterization of biomass feedstock, pretreatment and fractionation of biomass, and describes the process for conversion of biomass to value-added commodities. The authors bring biomass transformational strategies that are yet to be explored to the forefront, making this innovative book useful for graduate students and researchers in academia, government, and industry.

Bioprocessing for Biofuel Production

Bioprocessing for Biofuel Production
Strategies to Improve Process Parameters

by Neha Srivastava,Manish Srivastava,P.K. Mishra,Vijai Kumar Gupta

  • Publisher : Springer Nature
  • Release : 2020-10-28
  • Pages : 231
  • ISBN : 9811570701
  • Language : En, Es, Fr & De
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Converting biomass to biofuels involves hydrolyzing cellulose to sugars using cost-intensive commercial enzymes – an expensive step that makes large-scale production economically non-viable. As such, there is a need for low-cost bioprocessing. This book critically evaluates the available bioprocessing technologies for various biofuels, and presents the latest research in the field. It also highlights the recent developments, current challenges and viable alternative approaches to reduce the overall cost of producing biofuels.

Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power

Extremophilic Microbial Processing of Lignocellulosic Feedstocks to Biofuels, Value-Added Products, and Usable Power
A Book

by Rajesh K. Sani,Navanietha Krishnaraj Rathinam

  • Publisher : Springer
  • Release : 2018-07-02
  • Pages : 308
  • ISBN : 3319744593
  • Language : En, Es, Fr & De
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This book presents a review and in-depth analyses of improved biotechnological processes emphasizing critical aspects and challenges of lignocellulosic biomass conversion into biofuels and value-added products especially using extremophiles and recombinant microorganisms. The book specifically comprises extremophilic production of liquid and gaseous biofuels (bioethanol, biobutanol, biodiesel, biohydrogen, and biogas) as well as value added products (e.g. single cell protein, hydrocarbons, lipids, exopolysaccharides, and polyhydroxyalkanoates). The book also provides the knowledge on how to develop safe, more efficient, sustainable, and economical integrated processes for enhanced conversion of lignocellulosic feedstocks to liquid and gaseous biofuels. Finally the book describes how to perform the techno-economical and life-cycle assessments of new integrated processes involving extremophiles. These modeling exercises are critical in addressing any deficiencies associated with the demonstration of an integrated biofuels and value-added products production process at pilot scale as well as demonstration on the commercialization scale.

Metabolic Engineering of Zymomonas Mobilis for Improved Production of Ethanol from Lignocelluloses

Metabolic Engineering of Zymomonas Mobilis for Improved Production of Ethanol from Lignocelluloses
A Book

by Manoj Agrawal

  • Publisher : Unknown Publisher
  • Release : 2012
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Ethanol from lignocellulosic biomass is a promising alternative to rapidly depleting oil reserves. However, natural recalcitrance of lignocelluloses to biological and chemical treatments presents major engineering challenges in designing an ethanol conversion process. Current methods for pretreatment and hydrolysis of lignocelluloses generate a mixture of pentose (C5) and hexose (C6) sugars, and several microbial growth inhibitors such as acetic acid and phenolic compounds. Hence, an efficient ethanol production process requires a fermenting microorganism not only capable of converting mixed sugars to ethanol with high yield and productivity, but also having high tolerance to inhibitors. Although recombinant bacteria and yeast strains have been developed, ethanol yield and productivity from C5 sugars in the presence of inhibitors remain low and need to be further improved for a commercial ethanol production. The overarching objective of this work is to transform Zymomonas mobilis into an efficient whole-cell biocatalyst for ethanol production from lignocelluloses. Z. mobilis, a natural ethanologen, is ideal for this application but xylose (a C5 sugar) is not its 'natural' substrate. Back in 1995, researches at National Renewable Energy Laboratory (NREL) had managed to overcome this obstacle by metabolically engineering Z. mobilis to utilize xylose. However, even after more than a decade of research, xylose fermentation by Z. mobilis is still inefficient compared to that of glucose. For example, volumetric productivity of ethanol from xylose fermentation is 3- to 4- fold lower than that from glucose fermentation. Further reduction or complete inhibition of xylose fermentation occurs under adverse conditions. Also, high concentrations of xylose do not get metabolized completely. Thus, improvement in xylose fermentation by Z. mobilis is required. In this work, xylose fermentation in a metabolically engineered Z. mobilis was markedly improved by applying the technique of adaptive mutation. The adapted strain was able to grow on 10% (w/v) xylose and rapidly ferment xylose to ethanol within 2 days and retained high ethanol yield. Similarly, in mixed glucose-xylose fermentation, the strain produced a total of 9% (w/v) ethanol from two doses of 5% glucose and 5% xylose (or a total of 10% glucose and 10% xylose). Investigation was done to identify the molecular basis for efficient biocatalysis. An altered xylitol metabolism with reduced xylitol formation, increased xylitol tolerance and higher xylose isomerase activity were found to contribute towards improvement in xylose fermentation. Lower xylitol production in adapted strain was due to a single mutation in ZMO0976 gene, which drastically lowered the reductase activity of ZMO0976 protein. ZMO0976 was characterized as a novel aldo-keto reductase capable of reducing xylose, xylulose, benzaldehyde, furfural, 5-hydroxymethyl furfural, and acetaldehyde, but not glucose or fructose. It exhibited nearly 150-times higher affinity with benzaldehyde than xylose. Knockout of ZMO0976 was found to facilitate the establishment of xylose fermentation in Z. mobilis ZM4. Equipped with molecular level understanding of the biocatalytic process and insight into Z. mobilis central carbon metabolism, further genetic engineering of Z. mobilis was undertaken to improve the fermentation of sugars and lignocellulosic hydrolysates. These efforts culminated in construction of a strain capable of fermenting glucose-xylose mixture in presence of high concentration of acetic acid and another strain with a partially operational EMP pathway.

Fuel Processing and Energy Utilization

Fuel Processing and Energy Utilization
A Book

by Sonil Nanda,Prakash Kumar Sarangi,Dai-Viet N. Vo

  • Publisher : CRC Press
  • Release : 2019-02-22
  • Pages : 210
  • ISBN : 0429952376
  • Language : En, Es, Fr & De
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This book provides different aspects on fuel processing and refinery for energy generation. Most updated research findings along with case studies, real scenario examples, and extensive analyses of original research work and literature reviews is included in this book.

Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers

Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers
A Book

by Shang-Tian Yang,Hesham El-Ensashy,Nuttha Thongchul

  • Publisher : John Wiley & Sons
  • Release : 2013-05-24
  • Pages : 488
  • ISBN : 1118641949
  • Language : En, Es, Fr & De
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For researchers already familiar with biomass conversion technologies and for professionals in other fields, such as agriculture, food, and chemical industries, here is a comprehensive review of the emerging biorefinery industry. The book's content has been conveniently organized according to technologies (biomass feedstock and pretreatment, hydrolytic enzymes in biorefinery, and biofuels), with each chapter highlighting an important biobased industrial product. For undergraduate and graduate students, the book is a thorough introduction to biorefinery technologies.

Advances in Biofeedstocks and Biofuels, Liquid Biofuel Production

Advances in Biofeedstocks and Biofuels, Liquid Biofuel Production
A Book

by Lalit Kumar Singh,Gaurav Chaudhary

  • Publisher : John Wiley & Sons
  • Release : 2019-05-16
  • Pages : 408
  • ISBN : 1119459850
  • Language : En, Es, Fr & De
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Biofuels production is one of the most extensively studied fields in the energy sector that can provide an alternative energy source and bring the energy industry closer to sustainability. Biomass-based fuel production, or renewable fuels, are becoming increasingly important as a potential solution for man-made climate change, depleted oil reserves, and the dangers involved with hydraulic fracturing (or "fracking"). The price of oil will always be volatile and changeable, and, so long as industry and private citizens around the world need energy, there will be a need for alternative energy sources. The area known as "biofuels and biofeedstocks" is one of the most important and quickly growing pieces of the "energy pie." Biofuels and biofeedstocks are constantly changing, and new processes are constantly being created, changed, and improved upon. The area is rapidly changing and always innovative. It is important, therefore, that books like the volumes in this series are published and the information widely disseminated to keep the industry informed of the state-of-the-art. This third volume in the Advances in Biofeedstocks and Biofuels series focuses on the production of liquid biofuel, covering all of the major biofuels, such as biodiesel, biobutanol, bioethanol, and others. This engaging text touches on all of the most important new processes and technologies, providing the most up-to-date coverage of the science available to industry. It is a must-have for any engineer or scientist working with biofuel technology.

Lignocellulosic Ethanol Production from a Biorefinery Perspective

Lignocellulosic Ethanol Production from a Biorefinery Perspective
Sustainable Valorization of Waste

by Deepansh Sharma,Anita Saini

  • Publisher : Springer Nature
  • Release : 2020-07-20
  • Pages : 227
  • ISBN : 9811545731
  • Language : En, Es, Fr & De
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This book provides an overview of the multi-dimensional approach for the production of ethanol from lignocellulosic biomass. The sustainability of this biofuel, the current and future status of the technology and its role in waste valorization are also addressed. Bioethanol from lignocellulosic material has emerged as an alternative to the traditional first-generation bioethanol. The book also discusses various pretreatment methods for effective separation of the various components of lignocellulosic feedstock as well as their advantages, and limitations. It describes the valorization of lignocellulosic waste through the production of bioethanol and emphasizes the significance of waste utilization in managing the production cost of the fuel. Finally, the utilization of genetically engineered plants and microorganisms to increase the conversion efficiency is reviewed.

Waste to Energy: Prospects and Applications

Waste to Energy: Prospects and Applications
A Book

by Brijendra Kumar Kashyap,Manoj Kumar Solanki,Dev Vrat Kamboj,Akhilesh Kumar Pandey

  • Publisher : Springer Nature
  • Release : 2021-03-12
  • Pages : 438
  • ISBN : 9813343478
  • Language : En, Es, Fr & De
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This book addresses waste generation problems from various sectors, including industries, agriculture, and household. It focuses on how modern biotechnological approaches could help manage waste in an eco-friendly manner and generate precious bioenergy. It discusses the inadequate waste management systems damaging the environment and its adverse impacts on climate change-related problems. This book covers all the essential information regarding various types of waste and their management. It is a comprehensive compilation for understanding the efficient generation of bioenergy. It is a relevant reading material (resource) for anyone who wishes to study waste management as Chemist, Biologist, Biotechnologist, Industrialist, Ecologist, Microbiologist, Economist, and all disciplines related to the environment.

Microbial Technologies in Advanced Biofuels Production

Microbial Technologies in Advanced Biofuels Production
A Book

by Patrick C. Hallenbeck

  • Publisher : Springer Science & Business Media
  • Release : 2011-12-16
  • Pages : 274
  • ISBN : 1461412080
  • Language : En, Es, Fr & De
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Concerns over dwindling fossil fuel reserves and impending climate changes have focused attention worldwide on the need to discover alternative, sustainable energy sources and fuels. Biofuels, already produced on a massive industrial scale, are seen as one answer to these problems. However, very real concerns over the effects of biofuel production on food supplies, with some of ht recent increases in worldwide food costs attributable to biofuel production, have lead to the realization that new, non-food substrates for biofuel production must be bought online. This book is an authoritative, comprehensive, up-to-date review of the various options under development for the production of advanced biofuels as alternative energy sources. A general overview and introductory chapters for each section place the field in the context as well as provide essential basic notions for the more general reader. Accomplished, internationally recognized experts carrying out research on individual focus areas contribute specific technical chapters detailing present progress and future prospects.

Systems Metabolic Engineering

Systems Metabolic Engineering
A Book

by Christoph Wittmann,Sang Yup Lee

  • Publisher : Springer Science & Business Media
  • Release : 2012-06-15
  • Pages : 388
  • ISBN : 9400745346
  • Language : En, Es, Fr & De
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Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Lignocellulosic Biomass to Liquid Biofuels

Lignocellulosic Biomass to Liquid Biofuels
A Book

by Abu Yousuf,Filomena Sannino,Domenico Pirozzi

  • Publisher : Academic Press
  • Release : 2019-11-20
  • Pages : 358
  • ISBN : 0128162805
  • Language : En, Es, Fr & De
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Lignocellulosic Biomass to Liquid Biofuels explores the existing technologies and most recent developments for the production of second generation liquid biofuels, providing an introduction to lignocellulosic biomass and the processes for its conversion into biofuels. The book demonstrates biorefinery concepts compared with petro refinery, as well as the challenges of second generation biofuels processing. In addition to current pre-treatment techniques and their technical, environmental and economic implications, chapters included also further examine the particularities of conversion processes for bioethanol, biobutanol and biodiesel through chemical, biochemical and combined approaches. Finally, the book looks into concepts and tools for techno-economic and environmental analysis, which include supply chain assessment, by-products, zero-waste techniques and process evaluation and optimization. Lignocellulosic Biomass to Liquid Biofuels is particularly useful for researchers in the field of liquid biofuels seeking alternative chemical and biochemical pathways or those interested advanced methods to calculate maximum yield for each process and methods to simulate the implications and costs of scaling up. Furthermore, with the introduction provided by this volume, researchers and graduate students entering the field will be able to quickly get up to speed and identify knowledge gaps in existing and upcoming technology the book’s comprehensive overview. Examines the state-of-the-art technology for liquid biofuels production from lignocellulosic biomass Provides a comprehensive overview of the existing chemical and biochemical processes for second generation biofuel conversion Presents tools for the techno-economic and environmental analysis of technologies, as well as for the scale-up simulation of conversion processes