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Human Stem Cell Manual

Human Stem Cell Manual
A Laboratory Guide

by Jeanne F. Loring,Suzanne Peterson

  • Publisher : Academic Press
  • Release : 2012
  • Pages : 619
  • ISBN : 0123854733
  • Language : En, Es, Fr & De
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This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses. • Offers a comprehensive introduction to stem cell biology and culture for medical and biology researchers investigating diagnostics and treatments for various diseases • Presents a historical introduction, discussion of alternative methods, and common pitfalls for basic and advanced experimental strategies • Includes new chapters devoted to iPS cells and other alternative sources for generating human stem cells written by the scientists who made these breakthroughs

Human Stem Cell Technology and Biology

Human Stem Cell Technology and Biology
A Research Guide and Laboratory Manual

by Gary S. Stein,Maria Borowski,Mai X. Luong,Meng-Jiao Shi,Kelly P. Smith,Priscilla Vazquez

  • Publisher : John Wiley & Sons
  • Release : 2011-03-04
  • Pages : 440
  • ISBN : 9780470889893
  • Language : En, Es, Fr & De
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Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Human Stem Cell Manual

Human Stem Cell Manual
A Laboratory Guide

by Suzanne Peterson,Jeanne F. Loring,Robin L. Wesselschmidt,Philip H. Schwartz

  • Publisher : Elsevier
  • Release : 2011-10-10
  • Pages : 488
  • ISBN : 0080549888
  • Language : En, Es, Fr & De
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Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.

A Manual for Primary Human Cell Culture

A Manual for Primary Human Cell Culture
A Book

by Kee Woei Ng,Jan-Thorsten Schantz

  • Publisher : World Scientific
  • Release : 2010
  • Pages : 188
  • ISBN : 9812834788
  • Language : En, Es, Fr & De
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This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Hematopoietic Stem Cells

Hematopoietic Stem Cells
The Mulhouse Manual

by Eckart W. Wunder

  • Publisher : Alphamed Press
  • Release : 1994
  • Pages : 320
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Preservation of Cells

Preservation of Cells
A Practical Manual

by Allison Hubel

  • Publisher : John Wiley & Sons
  • Release : 2018-02-13
  • Pages : 216
  • ISBN : 1118989848
  • Language : En, Es, Fr & De
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Helps those that use cell preservation to develop new protocols or improve existing protocols This book provides readers with the tools needed to develop or debug a preservation protocol for cells. The core structure and content of the text grew from a professional short course that has been offered at the Biopreservation Core Resource for the last 10 years. This comprehensive text describes, step by step, the individual elements of a protocol, including the relevant scientific principles for each phase of the protocol. It can be used by anyone who is involved in cell preservation—even by those who are not experts in freezing of cells—because it provides the scientific basis for those that want to understand the basis for the protocol. Preservation of Cells: A Practical Manual begins by first introducing readers to the subject of preserving cells. It then goes on to cover Pre-freeze Processing and Characterization; Formulation and Introduction of Cryopreservation Solutions; Freezing Protocols; Storage and Shipping of Frozen Cells; Thawing and Post Thaw Processing; Post-thaw Assessment; and Algorithm-driven Protocol Optimization. Clearly explains the reasons behind every step in the development of a preservation protocol and the scientific principles behind them Provides alternative modes of preservation for when conventional methods of cryopreservation are not appropriate for a given cell type or application Enables more organization to achieve improved post thaw recoveries and process consistency Preservation of Cells: A Practical Manual is an important book for researchers, laboratory technicians and students in cell biology, stem cell biology, tissue engineering, and regenerative medicine. It is also useful to cell bankers, regenerative medicine, biomarker discovery or precision medicine companies, and cell therapy labs, blood bankers, biobankers, and biotechnology companies.

Culture of Animal Cells

Culture of Animal Cells
A Manual of Basic Technique and Specialized Applications

by R. Ian Freshney

  • Publisher : John Wiley & Sons
  • Release : 2011-02-02
  • Pages : 768
  • ISBN : 9780470649350
  • Language : En, Es, Fr & De
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This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

A Manual for Primary Human Cell Culture

A Manual for Primary Human Cell Culture
A Book

by Kee Woei Ng,Jan-Thorsten Schantz

  • Publisher : World Scientific Publishing Company Incorporated
  • Release : 2010
  • Pages : 188
  • ISBN : 9789812834775
  • Language : En, Es, Fr & De
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This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Manual For Primary Human Cell Culture, A (2nd Edition)

Manual For Primary Human Cell Culture, A (2nd Edition)
A Book

by Schantz Jan-thorsten,Ng Kee Woei

  • Publisher : World Scientific Publishing Company
  • Release : 2010-01-04
  • Pages : 212
  • ISBN : 9813101210
  • Language : En, Es, Fr & De
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This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Atlas of Human Pluripotent Stem Cells in Culture

Atlas of Human Pluripotent Stem Cells in Culture
A Book

by Lyn Healy,Ludmila Ruban

  • Publisher : Springer
  • Release : 2014-11-07
  • Pages : 206
  • ISBN : 1489975071
  • Language : En, Es, Fr & De
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This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.

Culture of Human Stem Cells

Culture of Human Stem Cells
A Book

by R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach

  • Publisher : John Wiley & Sons
  • Release : 2007-07-16
  • Pages : 360
  • ISBN : 9780470167519
  • Language : En, Es, Fr & De
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This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Bone Marrow and Stem Cell Processing

Bone Marrow and Stem Cell Processing
A Manual of Current Techniques

by Hans Joachim Deeg,Ronald A. Sacher

  • Publisher : F A Davis Company
  • Release : 1992
  • Pages : 487
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Embryonic Stem Cells

Embryonic Stem Cells
Differentiation and Pluripotent Alternatives

by Michael S. Kallos

  • Publisher : BoD – Books on Demand
  • Release : 2011-10-12
  • Pages : 520
  • ISBN : 9533076321
  • Language : En, Es, Fr & De
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The ultimate clinical implementation of embryonic stem cells will require methods and protocols to turn these unspecialized cells into the fully functioning cell types found in a wide variety of tissues and organs. In order to achieve this, it is necessary to clearly understand the signals and cues that direct embryonic stem cell differentiation. This book provides a snapshot of current research on the differentiation of embryonic stem cells to a wide variety of cell types, including neural, cardiac, endothelial, osteogenic, and hepatic cells. In addition, induced pluripotent stem cells and other pluripotent stem cell sources are described. The book will serve as a valuable resource for engineers, scientists, and clinicians as well as students in a wide range of disciplines.

Human Adult Stem Cells

Human Adult Stem Cells
A Book

by John Masters,Bernhard Ø Palsson

  • Publisher : Springer Science & Business Media
  • Release : 2009-05-30
  • Pages : 208
  • ISBN : 9048122694
  • Language : En, Es, Fr & De
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The aim of volume 7 of Human Cell Culture is to provide clear and precise methods for growing primary cultures of adult stem cells from various human tissues and describe culture conditions in which these adult stem cells differentiate along their respective lineages. The book will be of value to biomedical scientists and of special interest to stem cell biologists and tissue engineers. Each chapter is written by experts actively involved in growing human adult stem cells.

Stem Cells and Cancer Stem Cells, Volume 13

Stem Cells and Cancer Stem Cells, Volume 13
Therapeutic Applications in Disease and Injury

by M.A. Hayat

  • Publisher : Springer
  • Release : 2015-10-26
  • Pages : 123
  • ISBN : 9401772339
  • Language : En, Es, Fr & De
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This is the thirteenth volume in a series which presents current understanding of stem cells and cancer stem cells. This book presents many relevant topics including the different sources of cancer stem cells: the features, roles and clinical potential of stem cells emerge from engaging with this comprehensive work. Chapters explore molecular mechanisms underlying human somatic cell reprogramming to generate induced pluripotent stem cells and provide expert discussion of the advantages and limitations of applications of some of the stem cell types (pluripotent stem cells, neural stem cells). Many advances are explored, including the importance of stem cell markers in diagnosis, the role of stem cells in angiogenesis and a method for isolating multi potent endothelial-like cells from human adipose tissue, to name a few. Readers will also find a consideration of mathematical models and other quantitative tools which could facilitate research and discovery in the field. The editor’s preface to the book and the series is particularly helpful in introducing the work presented in this volume. The detailed overviews, practical experience and insights of international expert authors presented in this handbook will be of value to both professional practitioners and scholars in basic research.

Encyclopedia of Stem Cell Research

Encyclopedia of Stem Cell Research
A Book

by Anonim

  • Publisher : SAGE
  • Release : 2008
  • Pages : 902
  • ISBN : 141295908X
  • Language : En, Es, Fr & De
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"Provides an understanding of the basic concepts in stem cell biology and addresses the politics, ethics, and challenges currently facing the field"--From publisher description.

Freshney's Culture of Animal Cells

Freshney's Culture of Animal Cells
A Manual of Basic Technique and Specialized Applications

by R. Ian Freshney,Amanda Capes-Davis

  • Publisher : John Wiley & Sons
  • Release : 2021-03-17
  • Pages : 832
  • ISBN : 1119513049
  • Language : En, Es, Fr & De
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FRESHNEY’S CULTURE OF ANIMAL CELLS THE NEW EDITION OF THE LEADING TEXT ON THE BASIC METHODOLOGY OF CELL CULTURE, FULLY UPDATED TO REFLECT NEW APPLICATIONS INCLUDING IPSCS, CRISPR, AND ORGAN-ON-CHIP TECHNOLOGIES Freshney’s Culture of Animal Cells is the most comprehensive and up-to-date resource on the principles, techniques, equipment, and applications in the field of cell and tissue culture. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more. The eighth edition contains extensively revised material that reflects the latest techniques and emerging applications in cell culture, such as the use of CRISPR/Cas9 for gene editing and the adoption of chemically defined conditions for stem cell culture. A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice. Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing. This landmark text: Provides comprehensive single-volume coverage of basic skills and protocols, specialized techniques and applications, and new and emerging developments in the field Covers every essential area of animal cell culture, including lab design, disaster and contingency planning, safety, bioethics, media preparation, primary culture, mycoplasma and authentication testing, cell line characterization and cryopreservation, training, and troubleshooting Features a wealth of new content including protocols for gene delivery, iPSC generation and culture, and tumor spheroid formation Includes an updated and expanded companion website containing figures, artwork, and supplementary protocols to download and print The eighth edition of Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field, including undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories.

Stem Cells Handbook

Stem Cells Handbook
A Book

by Stewart Sell

  • Publisher : Springer Science & Business Media
  • Release : 2013-08-16
  • Pages : 534
  • ISBN : 1461476968
  • Language : En, Es, Fr & De
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This book discusses critical areas of progress in stem cell research, including the most recent research and applications of pluripotent embryonic cells, induced pluripotent cells, oligopotent tissue stem cells and cancer stem cells. The text covers basic knowledge of stem cell biology, stem cell ethics, development of techniques for applying stem cell therapy, the technology of obtaining appropriate cells for transplantation as well as the role of stem cells in cancer and how therapy may be directed to cancer stem cells. This new volume is essential reading for all scientists currently in the field or allied research areas, and those for those graduate students who envision a career in stem cells.

Biobanking and Cryopreservation of Stem Cells

Biobanking and Cryopreservation of Stem Cells
A Book

by Feridoun Karimi-Busheri,Michael Weinfeld

  • Publisher : Springer
  • Release : 2016-11-10
  • Pages : 238
  • ISBN : 3319454579
  • Language : En, Es, Fr & De
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Biobanking is considered to be one of the ten ideas changing the world with an estimated value of $45 billion by 2025. Despite the challenges, as the climate for innovation in the biobanking industry continues to flourish around the world, it is certain that amazing discoveries will emerge from this large-scale method of preserving and accessing human samples; biobanking is no longer just a place for collecting and storing samples. This book will cover a wide variety of subjects from across the future biobanking spectrum including scientific strategies, personalized medicine, regenerative medicine and stem cell challenges, disease surveillance, population genetics and innovative methods of biobanking.

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
A Book

by Awtar Krishan,H. Krishnamurthy,Satish Totey

  • Publisher : John Wiley and Sons
  • Release : 2011-05-12
  • Pages : 410
  • ISBN : 1118125851
  • Language : En, Es, Fr & De
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A much-needed primer on the use of laser flow cytometry for stemcell analysis Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker expression, cell cycle position, proliferation,and apoptosis. However, no resources specifically address the useof this methodology for the study of stem cells; this is especiallyimportant as stem cell analysis involves specialized methods andstaining procedures based on specific characteristics such asmarker expression, cell size, drug transport, and efflux of thestem cells. Now, this book reviews these procedures, discusses the sciencebehind them, and provides real-world examples to illustrate theusefulness of the methods. It brings together world-class expertsin pathology, biophysics, immunology, and stem cell research, whodraw upon their extensive experience with the methods and showexamples of good data to help guide researchers in the rightdirection. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stemcells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture andcardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavityfluids This book is an essential resource for all graduate students,practitioners in developing countries, libraries and bookrepositories of universities and research institutions, andindividual researchers. It is also of interest to laboratoriesengaged in stem cell research and use of stem cells for tissueregeneration, and to any organization dealing in stem cell andtissue regeneration research.