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Light and Video Microscopy

Light and Video Microscopy
A Book

by Randy O. Wayne

  • Publisher : Academic Press
  • Release : 2019-06-11
  • Pages : 510
  • ISBN : 0128161671
  • Language : En, Es, Fr & De
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Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. Provides a fully-revised, updated resource on three-dimensional (3D) structures Contains a new appendices on Diffraction Theory and Advanced Image Processing Provides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopy Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography

Light and Video Microscopy

Light and Video Microscopy
A Book

by Randy O. Wayne

  • Publisher : Academic Press
  • Release : 2013-12-16
  • Pages : 366
  • ISBN : 0124115365
  • Language : En, Es, Fr & De
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The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Video Microscopy

Video Microscopy
A Book

by Shinya Inoue

  • Publisher : Springer
  • Release : 2013-09-01
  • Pages : 584
  • ISBN : 9781475769272
  • Language : En, Es, Fr & De
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Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Video Microscopy

Video Microscopy
A Book

by Shinya Inoue,Robert J. Walter

  • Publisher : Springer
  • Release : 1986-05
  • Pages : 584
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Video Microscopy

Video Microscopy
A Book

by Anonim

  • Publisher : Academic Press
  • Release : 1998-04-10
  • Pages : 334
  • ISBN : 9780080859545
  • Language : En, Es, Fr & De
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Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Practical laboratory guide for methods and technologies used with video microscopy Comprehensive, easy-to-follow instructions February 1998, c. 334 pp.

Video Microscopy

Video Microscopy
A Book

by Shinya Inoue

  • Publisher : Springer Science & Business Media
  • Release : 2013-11-11
  • Pages : 584
  • ISBN : 1475769253
  • Language : En, Es, Fr & De
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Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Video Microscopy

Video Microscopy
The Fundamentals

by Shinya Inoué,Kenneth R. Spring

  • Publisher : Springer
  • Release : 1997-08-31
  • Pages : 742
  • ISBN : 9780306455315
  • Language : En, Es, Fr & De
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The current edition of Video Microscopy has been totally revised to reflect the advances in the tools for electronic imaging, processing, recording, and analysis, as well as applications that are being made in video microscopy and related modes of electronic imaging. The less spiral organization of the revised edition reflects an audience expected to be more experi enced in video and computer image processing than in the earlier days when coupling of video equipment and computers to the light microscope was still considered to be a novelty. Nevertheless, we have emphasi2;ed the how-tos, as well as the fundamental principles, involved in imaging and processing ih the light microscope, the human visual system, video and related electronic imaging, and the digital image processor in the hope that the reader will develop enough understanding, not only to apply rationally what is available, but also to contribute actively to the development of this evolving field. In the text, the terms appearing in the Glossary are printed in bold type, generally, at first appearance. Italics are used for emphasis.

Electronic Light Microscopy

Electronic Light Microscopy
The Principles and Practice of Video-Enhanced Contrast, Digital Intensified Fluorescence, and Confocal Scanning Light Microscopy

by David M. Shotton

  • Publisher : Wiley-Liss
  • Release : 1993-03-04
  • Pages : 368
  • ISBN : 9780471560777
  • Language : En, Es, Fr & De
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Uses a methods-oriented approach to examine the principles and practice of confocal and video microscopy, including computer reconstruction in three-dimensional fluorescence microscopy.

Virtual Microscopy and Virtual Slides in Teaching, Diagnosis, and Research

Virtual Microscopy and Virtual Slides in Teaching, Diagnosis, and Research
A Book

by Robert W. Ogilvie

  • Publisher : CRC Press
  • Release : 2005-06-22
  • Pages : 376
  • ISBN : 142003930X
  • Language : En, Es, Fr & De
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Despite a brief history, the technologies of virtual microscopy and virtual slides have captured the imagination of many, especially this current crop of students. Having come of age in the computer and Internet age, this emerging group of technicians and researchers tends to display a distinct preference for virtual slides and virtual microscopes.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
A Book

by James Pawley

  • Publisher : Springer Science & Business Media
  • Release : 2013-04-17
  • Pages : 632
  • ISBN : 1475753489
  • Language : En, Es, Fr & De
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This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Light Microscopy in Biology

Light Microscopy in Biology
A Practical Approach

by Alan J. Lacey

  • Publisher : OUP Oxford
  • Release : 1999-06-03
  • Pages : 474
  • ISBN : 0191565725
  • Language : En, Es, Fr & De
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Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Light Microscopy in Biology

Light Microscopy in Biology
A Practical Approach

by Alan J. Lacey

  • Publisher : Oxford University Press, USA
  • Release : 1999
  • Pages : 452
  • ISBN : 9780199636709
  • Language : En, Es, Fr & De
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Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that havebeen developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include:confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicineand cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Digital Microscopy

Digital Microscopy
A second edition of "Video Microscopy"

by Anonim

  • Publisher : Academic Press
  • Release : 2003-12-18
  • Pages : 544
  • ISBN : 9780080546032
  • Language : En, Es, Fr & De
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This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half. Shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy Contains 10 new chapters addressing developments over the last several years Covers basic principles, applications, and equipment Spcialized video microscopy techniques are covered

Vertebrate Paleontological Techniques: Volume 1

Vertebrate Paleontological Techniques: Volume 1
A Book

by Patrick Leiggi,Peter May

  • Publisher : Cambridge University Press
  • Release : 1994
  • Pages : 368
  • ISBN : 9780521459006
  • Language : En, Es, Fr & De
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Everything that amateur and professional fossil hunters will ever need to know about modern palaeontological techniques and practice.

Handbook of Analytical Techniques for Forensic Samples

Handbook of Analytical Techniques for Forensic Samples
Current and Emerging Developments

by Chaudhery Mustansar Hussain,Deepak Rawtani,Gaurav Pandey,Maithri Tharmavaram

  • Publisher : Elsevier
  • Release : 2020-11-28
  • Pages : 452
  • ISBN : 0128223014
  • Language : En, Es, Fr & De
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Handbook of Analytical Techniques for Forensic Samples: Current and Emerging Developments discusses in detail the current trends and latest analytical techniques and methods commonly employed in forensic analysis in order to ensure the proper facilitation of justice. This book is useful for readers who wish to stay updated on the latest trends in the forensic analysis of samples encountered at crime scenes. Technological advancements, such as biosensors, nanotechnology, and taggant technology have upped the level of analysis in forensic science. These emergent technologies, incorporated with existing analytical techniques, are leading to more precise, accurate, and specific examination of forensic samples. Lab-on-a-chip technology has also eased several kinds of on-site analyses done by investigating teams at different types of crime scenes. This book covers the evolution of forensic sample analysis as well as these emerging trends and new technologies. Includes an entire section of experimental exercises for self-teaching and key concept review Covers laboratory protocols used in forensic science laboratories for the analysis of various samples through different analytical techniques Condenses the many aspects of forensic analytical chemistry into a single resource with easy-to-understand language for everyone from students to practitioners

Understanding Light Microscopy

Understanding Light Microscopy
A Book

by Jeremy Sanderson

  • Publisher : John Wiley & Sons
  • Release : 2019-03-04
  • Pages : 848
  • ISBN : 1118696743
  • Language : En, Es, Fr & De
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Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Collected Works of Shinya Inou‚

Collected Works of Shinya Inou‚
Microscopes, Living Cells, and Dynamic Molecules(With DVD-ROM)

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2022
  • Pages : 129
  • ISBN : 9814476498
  • Language : En, Es, Fr & De
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Pathways of a Cell Biologist

Pathways of a Cell Biologist
Through Yet Another Eye

by Shinya Inoué

  • Publisher : Springer
  • Release : 2016-08-29
  • Pages : 258
  • ISBN : 9811009473
  • Language : En, Es, Fr & De
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This book is the autobiography of Shinya Inoué, tracing his life from childhood to the present. Though he has made many contributions to science, perhaps the most remarkable one involves the visualization of dynamics in living cells by means of a polarizing light microscope, an innovation that changed the face of cell biology. Addressing readers curious to know why and how he achieved such success, the story begins with a prologue describing the end of World War II and Inoué’s lifelong collaborator, the great cell biologist Katsuma Dan. Following the prologue, the author’s childhood and teenage experiences during World War II are described, before the focus shifts to his scientific career and personal life. The book not only offers important tips for young researchers, it will also help them develop a passion for science.

Colloidal Gold

Colloidal Gold
Principles, Methods, and Applications

by Anonim

  • Publisher : Elsevier
  • Release : 2012-12-02
  • Pages : 421
  • ISBN : 0323139019
  • Language : En, Es, Fr & De
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Among the first books to cover principles and methodology of colloidal gold in microscopy Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book Discusses problems and limitations of techniques Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins Preserving and localizing antigenicity; sectioning; high-resolution morphological localization Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation Applications to biomedical studies Colloidal gold in conjunction with video-enhanced microscopy

Digital Microscopy

Digital Microscopy
A Book

by Greenfield Sluder,David E. Wolf

  • Publisher : Academic Press
  • Release : 2013-08-07
  • Pages : 672
  • ISBN : 0124078923
  • Language : En, Es, Fr & De
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The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods