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Liquid Chromatography

Liquid Chromatography
Fundamentals and Instrumentation

by Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola

  • Publisher : Elsevier
  • Release : 2017-06-22
  • Pages : 808
  • ISBN : 0128093455
  • Language : En, Es, Fr & De
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Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography
A Book

by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan

  • Publisher : John Wiley & Sons
  • Release : 2011-09-20
  • Pages : 960
  • ISBN : 1118210395
  • Language : En, Es, Fr & De
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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

High Performance Liquid Chromatography & Capillary Electrophoresis

High Performance Liquid Chromatography & Capillary Electrophoresis
Principles and Practices

by Andrea Weston,Phyllis R. Brown

  • Publisher : Elsevier
  • Release : 1997-07-21
  • Pages : 280
  • ISBN : 9780080534169
  • Language : En, Es, Fr & De
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HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Liquid Chromatography

Liquid Chromatography
Applications

by Salvatore Fanali,Paul R. Haddad,Colin Poole,Marja-Liisa Riekkola

  • Publisher : Elsevier
  • Release : 2017-06-23
  • Pages : 838
  • ISBN : 0128093447
  • Language : En, Es, Fr & De
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Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Liquid Chromatography Detectors

Liquid Chromatography Detectors
A Book

by R.P.W. Scott

  • Publisher : Elsevier
  • Release : 1986-02-01
  • Pages : 270
  • ISBN : 9780080858364
  • Language : En, Es, Fr & De
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The renaissance of liquid chromatography took place in the late 1960's and early 1970's. The first edition of this book published in 1977 described the detectors that were available at that time and which provided a performance matching that of the contemporary equipment with which they were associated. It is interesting to note that the most popular detectors then (the UV detector, the refractometer detector, the fluorescence detector and the electrical conductivity detector) are still the most commonly used detectors nearly a decade later. Detector design, however, has changed very significantly over the intervening years. Modern high efficiency columns provide very narrow peaks and very fast separations, and thus the physical design of the detectors had to change to meet these new challenges. In 1977, there was little real understanding of the important role played by the detector in the overall function of the chromatographic system and although some of the factors were pointed out in the first edition of this book, in retrospect they appeared to be little understood. This second edition gives an entirely new presentation of the subject of liquid chromatography detectors. It contains sections dealing with the fundamental aspects of the interaction between columns and detectors and the interaction between ancillary equipment and the detector. It brings the reader up-to-date with new designs and novel detecting systems that have been developed since 1977 and extends significantly the subject of the association of the liquid chromatography detector with spectroscopic techniques. In particular the book now explores the association of liquid chromatography with nuclear magnetic resonance, infrared and atomic absorption spectrometry. This book not only gives a comprehensive treatment of the subject of liquid chromatographic detectors and provides a rational procedure for defining their performance and so permit valid comparisons, but also discusses detector performance in relation to the whole of the chromatographic system.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
Fundamental Principles and Practice

by W.J. Lough,I.W. Wainer

  • Publisher : CRC Press
  • Release : 1995-09-30
  • Pages : 288
  • ISBN : 9780751400762
  • Language : En, Es, Fr & De
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High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Introduction to high performance liquid chromatography

Introduction to high performance liquid chromatography
A Book

by R. Hamilton

  • Publisher : Springer
  • Release : 2011-10-17
  • Pages : 248
  • ISBN : 9789400959408
  • Language : En, Es, Fr & De
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Since the first edition of this book the major advances have been in column packings, where over ninety per cent of separations are now performed using chemically bonded microparticulate packings, and in instrumentation. The use of microprocessor control has brought about a rationalization of mobile phase delivery systems and in detectors, the introduction of electrochemical and spectrophotometric detection other than in the ultra-violet region, has widened the field of applications and the sensitivity of the technique. The use of ion pair chromatography has increased at the expense of ion-exchange and this together with the improvements in detectors has greatly increased the appli cation of the technique in the biomedical field. These advances are described together with the established methods to enable the beginner to carry out a satisfactory separation and to gain the experience necessary for the full exploitation of the technique. R. J. Hamilton P. A. Sewell Liverpool,1981 1 Introduction to high performance liquid chromatography 1. 1 Introduction Chromatography in its many forms is widely used as a separative and an analytical technique. Gas chromatography since its introduction by James and Martin [1] has been pre-eminent in the field. Uquid chromatography in the of paper, thin-layer, ion-exchange, and exclusion (gel permeation and gel form filtration) chromatography had not been able to achieve the same success, mainly because of the poor efficiences and the long analysis times arising from the low mobile phase flow rates.

Preparative Liquid Chromatography

Preparative Liquid Chromatography
A Book

by B.A. Bidlingmeyer

  • Publisher : Elsevier
  • Release : 1987-07-01
  • Pages : 340
  • ISBN : 9780080858418
  • Language : En, Es, Fr & De
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This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus. The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.

Applications of High Performance Liquid Chromatography

Applications of High Performance Liquid Chromatography
A Book

by S. Pryde,M.T. Gilbert

  • Publisher : Springer Science & Business Media
  • Release : 1979-01-31
  • Pages : 256
  • ISBN : 9780412142208
  • Language : En, Es, Fr & De
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Polymer Characterization by Liquid Chromatography

Polymer Characterization by Liquid Chromatography
A Book

by G. Glöckner

  • Publisher : Elsevier
  • Release : 1987-02-01
  • Pages : 585
  • ISBN : 9780080858371
  • Language : En, Es, Fr & De
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The main subject of this book is the characterization of plastics. To a high degree the properties of these polymers depend on the distribution of the molar mass and of other structural features, and small deviations frequently have a great effect. Therefore the characterization of polymers cannot be restricted to the determination of mean values but must yield information on these distributions. Using classical methods, the analytical fractionation of polymer homologues and structurally isomeric polymers is extremely time-consuming. Therefore, efficient chromatographic techniques are being increasingly employed in modern polymer characterization. In the first place, high-performance liquid chromatography is applied, in the form of size exclusion chromatography. It is also possible, however, to use other separation modes. More space is devoted to these other possibilities in this volume than is merited by their current range of applications, as the author believes that many separation problems will be solved by separation techniques of the non-exclusion type. Nevertheless, much emphasis is placed on size exclusion chromatography. Not only because of its current wide range of applications, but also because its relative importance, as a complement to other chromatographic techniques may even increase in the forthcoming years. This book is the first to cover all phenomena related to the above considerations. Starting with an introduction to basic liquid chromatography and to polymer science, it deals with the adsorption behaviour of polymers, with gradient techniques, with the kinetic band broadening in liquid chromatography, with instrumental features and packing materials. The book consists of four balanced sections and related information from about 1800 references is compiled in the tables. Some 250 figures and 30 tables will help give the reader a clear insight of the topics discussed. The book is aimed at helping the analyst or polymer chemist who is looking for information about chromatographic methods for the characterization of polymers.

Gradient Elution in Column Liquid Chromatography

Gradient Elution in Column Liquid Chromatography
Theory and Practice

by P. Jandera,J. Churácék

  • Publisher : Elsevier
  • Release : 1985-05-01
  • Pages : 509
  • ISBN : 9780080858340
  • Language : En, Es, Fr & De
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Gradient Elution in Column Liquid Chromatography

Liquid Chromatography

Liquid Chromatography
Chapter 7. Liquid–Solid Chromatography

by L.R. Snyder,J.W. Dolan

  • Publisher : Elsevier Inc. Chapters
  • Release : 2013-01-08
  • Pages : 520
  • ISBN : 0128071346
  • Language : En, Es, Fr & De
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Liquid-solid or normal-phase chromatography is employed for the separation of non-ionic solutes by adsorption onto the surface of an inorganic oxide stationary phase and displacement by competition with the components of the mobile phase. It has particular advantages for compound-class separations and the separation of diasteromers and geometrical isomers soluble in organic solvents. Common stationary phases for high-performance liquid chromatography include silica and chemically-bonded silica phases with 3-cyanopropyl, 3-aminopropyl, and spacer bonded propanediol groups. The use of eluotropic series for mobile phase selection are described as well as general strategies for method development.

Instrumental Liquid Chromatography

Instrumental Liquid Chromatography
A Practical Manual on High-Performance Liquid Chromatographic Methods

by N.A. Parris

  • Publisher : Elsevier
  • Release : 2000-04-01
  • Pages : 447
  • ISBN : 9780080858302
  • Language : En, Es, Fr & De
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Instrumental Liquid Chromatography

Instrumentation for High Performance Liquid Chromatography

Instrumentation for High Performance Liquid Chromatography
A Book

by J.F.K. Huber

  • Publisher : Elsevier
  • Release : 2011-09-22
  • Pages : 203
  • ISBN : 9780080858135
  • Language : En, Es, Fr & De
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Instrumentation for High Performance Liquid Chromatography

Retention and Selectivity in Liquid Chromatography

Retention and Selectivity in Liquid Chromatography
Prediction, Standardisation and Phase Comparisons

by R.M. Smith

  • Publisher : Elsevier
  • Release : 1995-01-13
  • Pages : 456
  • ISBN : 9780080858654
  • Language : En, Es, Fr & De
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This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte. The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated. Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given. Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

High-Performance Liquid Chromatography

High-Performance Liquid Chromatography
Advances and Perspectives

by Csaba Horváth

  • Publisher : Elsevier
  • Release : 2013-10-22
  • Pages : 358
  • ISBN : 1483261832
  • Language : En, Es, Fr & De
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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 2 presents the fundamental aspects of high-performance liquid chromatography, laboratory technique for chemical analysis with a wide range of applications. The book consists of three chapters discussing the optimization of the column and the operating conditions of the chromatographic system; use of polar adsorbents and nonpolar eluents; and reversed-phase chromatography, the main branch of high-performance liquid chromatography. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a good reference material.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
A Book

by Mary T. Gilbert

  • Publisher : Elsevier
  • Release : 2013-10-22
  • Pages : 396
  • ISBN : 1483281299
  • Language : En, Es, Fr & De
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High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques

Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques
A Book

by Paul H. Gamache

  • Publisher : John Wiley & Sons
  • Release : 2017-05-30
  • Pages : 544
  • ISBN : 0470937785
  • Language : En, Es, Fr & De
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The first book devoted exclusively to a highly popular, relatively new detection technique Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques presents a comprehensive review of CAD theory, describes its advantages and limitations, and offers extremely well-informed recommendations for its practical use. Using numerous real-world examples based on contributors’ professional experiences, it provides priceless insights into the actual and potential applications of CAD across a wide range of industries. Charged aerosol detection can be combined with a variety of separation techniques and in numerous configurations. While it has been widely adapted for an array of industrial and research applications with great success, it is still a relatively new technique, and its fundamental performance characteristics are not yet fully understood. This book is intended as a tool for scientists seeking to identify the most effective and efficient uses of charged aerosol detection for a given application. Moving naturally from basic to advanced topics, the author relates fundamental principles, practical uses, and applications across a range of industrial settings, including pharmaceuticals, petrochemicals, biotech, and more. Offers timely, authoritative coverage of the theory, experimental techniques, and end-user applications of charged aerosol detection Includes contributions from experts from various fields of applications who explore CAD’s advantages over traditional HPLC techniques, as well its limitations Provides a current theoretical and practical understanding of CAD, derived from authorities on aerosol technology and separation sciences Features numerous real-world examples that help relate fundamental properties and general operational variables of CAD to its performance in a variety of conditions Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques is a valuable resource for scientists who use chromatographic techniques in academic research and across an array of industrial settings, including the biopharmaceutical, biotechnology, biofuel, chemical, environmental, and food and beverage industries, among others.

Liquid Chromatography/Mass Spectrometry

Liquid Chromatography/Mass Spectrometry
Techniques and Applications

by Alfred L. Yergey,Charles G. Edmonds,Ivor A.S. Lewis,Marvin L. Vestal

  • Publisher : Springer Science & Business Media
  • Release : 2013-12-14
  • Pages : 306
  • ISBN : 148993605X
  • Language : En, Es, Fr & De
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This book is intended both to be an introduction to techniques and applications of liquid chromatography/mass spectrometry and to serve as a reference for future workers. When we undertook its writing, we chose not to cover the field, particularly applications, exhaustively. Rather we wished to produce a book that would be of use to people just beginning to use the technique as well as to more advanced practitioners. In this regard, we have sought to highlight techniques and applications that are of current importance, while not neglecting descriptions of approaches that may be of significance in the future. We hope that we have succeeded in this. At the same time we hope that the bibliography, with indexes classified by author and title, will make this book of value to those who may disagree with our emphasis. ACKNOWLEDGMENTS. One of us (C. G. E. ) wishes to acknowledge the en couragment of Professor J. A. McCloskey in undertaking this project. All four of us are grateful for the continuous and expert assistance of V. A. Edmonds in the preparation of the Bibliography. Alfred L. Y ergey Bethesda, Maryland Charles G. Edmonds Richland, Washington lvor A. S. Lewis London, England Marvin L. Vestal Houston, Texas v Contents 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 2. Direct Liquid Introduction Interfaces . . . . . . . . . . 5 . . . 2. 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 2. Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. 3. Specific DLI Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 1. Capillary Inlets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2. 3. 2. Diaphragm Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2. 3. 3. Nebulizing Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

High-Performance Liquid Chromatography of Peptides and Proteins

High-Performance Liquid Chromatography of Peptides and Proteins
Separation, Analysis, and Conformation

by Colin T. Mant,Robert S. Hodges

  • Publisher : CRC Press
  • Release : 2017-11-22
  • Pages : 960
  • ISBN : 1351440748
  • Language : En, Es, Fr & De
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This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.