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Recent Advancement in Microbial Biotechnology

Recent Advancement in Microbial Biotechnology
Agricultural and Industrial Approach

by Surajit De Mandal,Ajit Kumar Passari

  • Publisher : Academic Press
  • Release : 2021-08-15
  • Pages : 366
  • ISBN : 9780128220986
  • Language : En, Es, Fr & De
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The rapid increase in microbial resources along with the development of biotechnological methods has revolutionized the field of microbial biotechnology. Genome characterization methods and metagenomic approaches further illustrate the role of microorganisms in various fields of research. Recent advancement in Microbial Biotechnology: vol. 2 provides an overview on the recent application of the microorganisms in agricultural and industrial improvements. The purpose of this book is to integrate all these diverse areas of research in a common platform. Recent advancement in Microbial Biotechnology targets researchers from both academia and industry, professors and graduate students working in molecular biology, microbiology and biotechnology. Gives insight in the exploration of microbial diversity in different systems Addresses the novel approaches to study microbial diversity Provides understanding to the basics with advance information of microbial biotechnology Discusses the techniques used to examine microbial diversity with their applications and respective pros and cons Explores the importance of microbial genomes studies in industrial applications

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Crop Improvement through Microbial Biotechnology

by Ram Prasad,Sarvajeet Singh Gill,Narendra Tuteja

  • Publisher : Elsevier
  • Release : 2018-02-20
  • Pages : 496
  • ISBN : 0444639888
  • Language : En, Es, Fr & De
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Crop Improvement through Microbial Biotechnology explains how certain techniques can be used to manipulate plant growth and development, focusing on the cross-kingdom transfer of genes to incorporate novel phenotypes in plants, including the utilization of microbes at every step, from cloning and characterization, to the production of a genetically engineered plant. This book covers microbial biotechnology in sustainable agriculture, aiming to improve crop productivity under stress conditions. It includes sections on genes encoding avirulence factors of bacteria and fungi, viral coat proteins of plant viruses, chitinase from fungi, virulence factors from nematodes and mycoplasma, insecticidal toxins from Bacillus thuringiensis, and herbicide tolerance enzymes from bacteria. Introduces the principles of microbial biotechnology and its application in crop improvement Lists various new developments in enhancing plant productivity and efficiency Explains the mechanisms of plant/microbial interactions and the beneficial use of these interactions in crop improvement Explores various bacteria classes and their beneficial effects in plant growth and efficiency

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Recent Advances in Application of Fungi and Fungal Metabolites: Biotechnological Interventions and Futuristic Approaches

by Joginder Singh,Praveen Gehlot

  • Publisher : Elsevier
  • Release : 2020-06-25
  • Pages : 316
  • ISBN : 0128226234
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Advances in Application of Fungi and Fungal Metabolites: Biotechnological Interventions and Futuristic Approaches is an invaluable resource for researchers planning to work in applied biotechnological interventions and futuristic approaches to fungi and fungal metabolite utilization. Special emphasis is placed on new research relating to fungal-based recombinant DNA technology and genomics analysis which place yeasts and filamentous fungi at the forefront of various contemporary commercial applications. Written in an easy-to-follow language by active researchers, the book presents cutting-edge fungal biotechnological applications in a manner that is accessible to all. Introduces recent biotechnological interventions and futuristic approaches to fungi and their metabolites Elaborates on perspectives and diverse applications of harnessing the potential of fungi and fungal metabolites in biotechnology Describes traditional uses and modern practices of accessing the potential of fungi and their metabolites in solving future needs

Actinobacteria: Diversity and Biotechnological Applications

Actinobacteria: Diversity and Biotechnological Applications
New and Future Developments in Microbial Biotechnology and Bioengineering

by Bhim Pratap Singh,Vijai Kumar Gupta,Ajit Kumar Passari

  • Publisher : Elsevier
  • Release : 2018-02-01
  • Pages : 362
  • ISBN : 0444639950
  • Language : En, Es, Fr & De
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Actinobacteria: Diversity and Biotechnological Applications: New and Future Developments in Microbial Biotechnology and Bioengineering, a volume in the series New and Future Developments in Microbial Biotechnology and Bioengineering series, offers the latest on the biotechnology of Kingdom actinobacteria, covering unique niches like their endosphere, rhizospheric soil and contaminated sites, etc. The book also covers the bioactive secondary metabolites obtained from actinobacteria and describes the application of microorganism (Actinobacteria) in plant growth promotion and in environmental cleanup. Finally, the book describes the biocontrol aspects of actinobacteria and how they can control fungal phytopathogens and the production of secondary metabolites. Includes an overview of all types of actinobacteria, source and enzymatic activity Lists various bioengineering methods for the production of these enzymes Reviews numerous industrial applications of actinobacteria, i.e., crop improvement, removal of heavy metals, etc. Offers unique coverage of the application of actinobacteria in bioremediation processes Explores the plant growth promoting potential of endophytic actinobacteria Describes biosynthetic potential genes associated with actinobacterial genome

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Recent Advances in Application of Fungi and Fungal Metabolites: Environmental and Industrial Aspects

by Joginder Singh,Praveen Gehlot

  • Publisher : Elsevier
  • Release : 2020-06-16
  • Pages : 270
  • ISBN : 0128226226
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Recent Advances in Application of Fungi and Fungal Metabolites: Environmental and Industrial Aspects provides a comprehensive overview of recent development and applied aspects of fungi and its metabolites in environmental and industrial settings. Fungi and fungal metabolites have great prospects for developing new products in a wide range of sectors. Many fungal metabolites are environmentally friendly, clean, non‐toxic agents used for environmental management practices. This book offers a systems approach and provides a means to share the latest developments and advances about the exploitation of fungal products, including their vide uses in the field of environment and industry. Introduces the aspects and advances of fungi and fungal metabolites in environmental and industry perspectives Discusses the potential of fungi and its metabolites in environmental management Includes a description of traditional uses and the modern practices of harnessing the potential of fungi and its metabolites in solving environment issues Provides details about usage of fungi and its metabolites for environmental management and industrial purposes

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Microbial Biotechnology in Agro-environmental Sustainability

by Jay Shankar Singh,DP Singh

  • Publisher : Elsevier
  • Release : 2019-03-29
  • Pages : 329
  • ISBN : 0444642811
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Biotechnology in Agro-environmental Sustainability describes, in detail, the various roles of microbial resources in the management of crop diseases and how microbes can be used as a source of income for biomass and bioenergy production. In addition, the book covers microbial inoculants as bio-fertilizers to enhance crop productivity, along with degraded land restoration. Users will find the latest information in the field of microbial biotechnology and its further applications in bio-fertilizers, bio-pesticides, its generation as an alternative source of energy, restoration degraded and marginal lands, the mitigation of global warming gases, and more. Describes microbial biotechnology and its applications in sustainable agriculture Provides information on the use of a variety of microbes for crop production Outlines microbe-based separation techniques for the removal of metal contaminants from soil Describes the role of microbial agents in the generation of alternative sources of energy Includes microbial tools and technologies for the restoration of degraded and marginal lands, the mitigation of global warming gases, and the bioremediation of polluted sites

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Microbial Cellulase System Properties and Applications

by Vijai G. Gupta

  • Publisher : Elsevier
  • Release : 2016-11-15
  • Pages : 300
  • ISBN : 0444635157
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Cellulase System Properties and Applications covers the biochemistry of cellulase system, its mechanisms of action, and its industrial applications. Research has shed new light on the mechanisms of microbial cellulase production and has led to the development of technologies for production and applications of cellulose degrading enzymes. The biological aspects of processing of cellulosic biomass have become the crux of future research involving cellulases and cellulolytic microorganisms, as they are being commercially produced by several industries globally and are widely being used in food, animal feed, fermentation, agriculture, pulp and paper, and textile applications. The book discusses modern biotechnology tools, especially in the area of microbial genetics, novel enzymes, and new enzyme and the applications in various industries. As a professional reference, this new book is useful to all researchers working with microbial cellulase system, both academic institutions and industry-based research bodies, as well as to teachers, graduate, and postgraduate students with information on continuous developments in microbial cellulase system. The book provides an indispensable reference source for chemists, biochemical engineers/bioengineers, biochemists, biotechnologists and researchers who want to know about the unique properties of this microbe and explore its future applications. Compiles the latest developments made and currently undergoing in the area of microbial cellulase system Chapters are contributed from top researchers on this area around the globe Includes information related to almost all areas of microbial cellulase system Extensive cover of current industrial applications and discusses potential future applications

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Aspergillus System Properties and Applications

by Vijai Kumar Gupta

  • Publisher : Elsevier
  • Release : 2016-10-27
  • Pages : 314
  • ISBN : 0444635130
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Aspergillus System Properties and Applications provides information on emerging issues related to recent advancements in aspergillus research and its applications in bioprocess technology, chemical engineering, genome biology, molecular taxonomy, secondary and metabolite production, industrial process and biofuels/bioenergy research, and alternative fuel development. The book covers the various novel enzymes secreted by these fungi and their specific use in the food, textile, pulp and paper, biocellulosic ethanol production, and other industries. The book describes research and experimentation on aspergillus activity and directly connects them to their use in bioprocess technology, chemical engineering, bioremediation process, secondary metabolite production, pharmaceutical processes, protein production, industrial process, biofuels/bioenergy research, and alternative fuel development. Readers will find this book to be an indispensable resource for biotechnologists, biochemical engineers, biochemists, microbiologists, bioinformatics researchers, and other biologists who are interested in learning about the potential applications of these fungi. Compiles available, up-to-date information on recent developments made in the study of aspergillus system properties Contains global content from pioneering international authors Presents current research efforts and links them to various applications, including uses in foods, textiles, pulp and paper, and in biocellulosic ethanol production Provides an indispensable resource for biologists who are interested in learning about the potential applications of the fungi aspergillus

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Microbial Genes Biochemistry and Applications

by H.B Singh,Vijai G. Gupta,Sudisha Jogaiah

  • Publisher : Elsevier
  • Release : 2018-10-19
  • Pages : 254
  • ISBN : 0444635106
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Genes Biochemistry and Applications consolidates the most widely used genetic methods available, bringing together the fields of biochemistry, biotechnology, and microbiology. The chapters outlined give clear and concise direction on both standard and applied microbial genetic improvements, presenting undergraduates, postgraduates, and researchers with the latest developments in microbial gene technology. In addition, the book describes the background and usefulness of each experiment in question. All chapters covered in the book are derived from current peer-reviewed literature as accepted by the international scientific community. Compiles the latest developments made in the area of microbial gene systems Includes exhaustive information on almost all areas of microbial gene technology Relates microbial engineering and its direct application to the production of many useful compounds Written by an international team of authors and compiled by award winning editors

Microbial Biotechnology in Food and Health

Microbial Biotechnology in Food and Health
A Book

by Ramesh C. Ray

  • Publisher : Academic Press
  • Release : 2020-09-13
  • Pages : 308
  • ISBN : 0128227613
  • Language : En, Es, Fr & De
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Microbial Biotechnology in Food and Health Science, volume one in the Applied Biotechnology Reviews series, offers two unique sections within the theme of genomics and bioprocessing and the bioengineering of microorganisms in the role of food science and human health. This volume provides review articles as the basis supporting biotechnological research useful to a wide scope of research initiatives. Important relevant information on genomics, proteomics and metabolomics are included as well as the emerging interdisciplinary area of synthetic biology which enables the metabolic engineering of microorganisms to produce pharmaceuticals. Applied Biotechnology Reviews is a series aimed at bringing all aspects of biotechnology as it is applied to food science – from agriculture through product processing into focus through topical volumes. Each volume will cover a relevant application approach in industrial biotechnology. Covers the latest biotechnological research articles on applications of microbes for food and health science Presents research articles to emphasize research methods and techniques useful for research outcomes Analysis detoxification properties of microorganisms in foods Includes methods of bioengineering of microbes to improve human insulin synthesis/recombinant protein

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Microbes in Soil, Crop and Environmental Sustainability

by Jay Shankar Singh

  • Publisher : Elsevier
  • Release : 2019-07-18
  • Pages : 386
  • ISBN : 0128182598
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Microbes in Soil, Crop and Environmental Sustainability reviews the exploitation of microbial biodiversity in soil with respect to nutrient-use efficiency, also discussing the improvement and maintenance of certain physical and chemical conditions in soil that can provide economic and environmental benefits toward agricultural sustainability. The utilization of microbes ranges from applications in biotechnology, marginal land restoration, the formulation of microbial inoculants, the enhancement of crop productivity, and the mitigation of global warming gases. Finally, various uses for microbial resources in crop disease management, bioenergy production, and income based on microbial cultivation are explored. Highlights the developments and achievements of microbial resources and their role in the sustainable management of soil fertility and agriculture productivity Outlines the role of microbial resource and biotechnology in sustainability to industry, agriculture, forest and management of environment Provides up-to-date information on the application of microbial resources and the role of biotechnology to meet the ever increasing demand of food, soil and plant productivity management Outlines enhancement in productivity through interventions of microbial bio-agents and eco-friendly technology

Microbial Biotechnology: Basic Research and Applications

Microbial Biotechnology: Basic Research and Applications
A Book

by Joginder Singh

  • Publisher : Springer Nature
  • Release : 2021
  • Pages : 329
  • ISBN : 9811528179
  • Language : En, Es, Fr & De
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Advances in Microbial Biotechnology

Advances in Microbial Biotechnology
Current Trends and Future Prospects

by Pradeep Kumar, PhD.,Jayanta Kumar Patra,Pranjal Chandra

  • Publisher : CRC Press
  • Release : 2018-10-03
  • Pages : 574
  • ISBN : 1351248898
  • Language : En, Es, Fr & De
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Over the last few decades, the rapid and vast development of advanced microbial bioresources and metagenomics techniques has completely transformed the field of microbial biotechnology. Our understanding of microbial diversity, evolutionary biology, and microbial interaction with their animal and plant hosts at molecular level has been revolutionized with an abundance of new research. This new volume, Advances in Microbial Biotechnology: Current Trends and Future Prospect, focuses on the application of microorganisms for several purposes: for plant protection and improvement, for environmental remediation purposes, and for the improvement of human health. Various applications of microorganisms are covered broadly and have been appropriately reflected in depth in different chapters. The book is divided into four major sections: applied microbiology in agriculture microbes in the environment microbes in human health microbes in nanotechnology The book provides insight into the diverse microorganisms that have been explored and exploited in the development of various applications for agricultural improvements. The book also looks at the application of microbes for the removal of pollutants and the recovery of metals and oils. Also discussed is the detection and exploitation of microorganisms in the diagnosis of human diseases, providing possible holistic approaches to health. This new volume will provide a wealth of information on new research on the application of microbial biotechnology today.

Recent Advancement in White Biotechnology Through Fungi

Recent Advancement in White Biotechnology Through Fungi
Volume 2: Perspective for Value-Added Products and Environments

by Ajar Nath Yadav,Sangram Singh,Shashank Mishra,Arti Gupta

  • Publisher : Springer
  • Release : 2019-04-24
  • Pages : 504
  • ISBN : 3030148467
  • Language : En, Es, Fr & De
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White biotechnology is industrial biotechnology dealing with various biotech products through applications of microbes. The main application of white biotechnology is commercial production of various useful organic substances, such as acetic acid, citric acid, acetone, glycerine, etc., and antibiotics like penicillin, streptomycin, mitomycin, etc., and value added product through the use of microorganisms especially fungi and bacteria. The value-added products included bioactive compounds, secondary metabolites, pigments and industrially important enzymes for potential applications in agriculture, pharmaceuticals, medicine and allied sectors for human welfare. In the 21st century, techniques were developed to harness fungi to protect human health (through antibiotics, antimicrobial, immunosuppressive agents, value-added products etc.), which led to industrial scale production of enzymes, alkaloids, detergents, acids, biosurfactants. The first large-scale industrial applications of modern biotechnology have been made in the areas of food and animal feed production (agricultural/green biotechnology) and pharmaceuticals (medical/red biotechnology). In contrast, the production of bio-active compounds through fermentation or enzymatic conversion is known industrial or white biotechnology. The beneficial fungal strains may play important role in agriculture, industry and the medical sectors. The beneficial fungi play a significance role in plant growth promotion, and soil fertility using both, direct (solubilization of phosphorus, potassium and zinc; production of indole acetic acid, gibberellic acid, cytokinin and siderophores) and indirect (production of hydrolytic enzymes, siderophores, ammonia, hydrogen cyanides and antibiotics) mechanisms of plant growth promotion for sustainable agriculture. The fungal strains and their products (enzymes, bio-active compounds and secondary metabolites) are very useful for industry. The discovery of antibiotics is a milestone in the development of white biotechnology. Since then, white biotechnology has steadily developed and now plays a key role in several industrial sectors, providing both high valued nutraceuticals and pharmaceutical products. The fungal strains and bio-active compounds also play important role in the environmental cleaning. This volume covers the latest research developments related to value-added products in white biotechnology through fungi.

Recent Developments in Applied Microbiology and Biochemistry

Recent Developments in Applied Microbiology and Biochemistry
Volume 2

by Buddolla Viswanath

  • Publisher : Academic Press
  • Release : 2020-10-16
  • Pages : 380
  • ISBN : 0128214074
  • Language : En, Es, Fr & De
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Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a comprehensive treatment and understanding on application oriented microbial concepts, giving readers insights into recent developments in microbial biotechnology and medical, agricultural and environmental microbiology. Discusses microbial proteome analyses and their importance in medical microbiology Explores emerging trends in the prevention of current global health problems, such as cancer, obesity and immunity Shows recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomics approaches Guides readers through the status and recent developments in analytical methods for the detection of foodborne microorganisms

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Recent Advances in Application of Fungi and Fungal Metabolites: Current Aspects

by Joginder Singh,Praveen Gehlot

  • Publisher : Elsevier
  • Release : 2020-09-21
  • Pages : 302
  • ISBN : 0128225572
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering presents an account of recent developments and applied aspects of fungi and its metabolites for human welfare. The fungi and its metabolites are employed in diverse fields of agri-food, biochemistry, chemical engineering, diagnostics, pharmaceuticals and medical device development. The book contains chapters by the eminent researchers working with fungi and fungal metabolites who explain their importance and potential in manifold prospects. The book includes a description of various fungal metabolites and their chemistry and biotechnology. Highlights the latest developments surrounding the utilization of fungi and fungal metabolites Overviews applied aspects of fungi and their metabolites for human welfare Details the usage of fungi and their metabolites in diverse fields Identifies the importance and potential of fungi and fungal metabolites in manifold prospects Illustrates recent trends in fungal metabolite research using elaborate, expressive tables and figures with concise information

Microbial Biotechnology: Basic Research and Applications

Microbial Biotechnology: Basic Research and Applications
A Book

by Joginder Singh,Ashish Vyas,Shanquan Wang,Ram Prasad

  • Publisher : Springer
  • Release : 2020-07-15
  • Pages : 370
  • ISBN : 9789811528163
  • Language : En, Es, Fr & De
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M​icrobial biotechnology is an important area that promotes advanced research into using microbes for value-added products, human nutrition, and the overall wellbeing of society. This book presents the latest information on the use of microbes for sustainable development, and highlights state-of-the-art biotechnological techniques used to harness microbial biotechnological traits on a commercial scale. Gathering contributions from authoritative researchers in the field, it addresses recent advances in microbial biotechnological approaches that offer sustainable options for future generations. Exploring a broad range of microbial products and their uses, the book specifically places emphasis on the application of microorganisms in healthcare, the environment and industry. It also discusses various compound classes derived from microbial metabolites. Pursuing a holistic approach to recent advances in the utilization of various microbes as biotechnological tools, the book also covers traditional uses, and explores emerging strategies to harness their full potential. Accordingly, it offers a valuable resource for researchers and graduate students alike.

Advances in Microbial Biotechnology

Advances in Microbial Biotechnology
Current Trends and Future Prospects

by Pradeep Kumar, PhD.,Jayanta Kumar Patra,Pranjal Chandra

  • Publisher : CRC Press
  • Release : 2018-10-03
  • Pages : 574
  • ISBN : 1351248901
  • Language : En, Es, Fr & De
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Over the last few decades, the rapid and vast development of advanced microbial bioresources and metagenomics techniques has completely transformed the field of microbial biotechnology. Our understanding of microbial diversity, evolutionary biology, and microbial interaction with their animal and plant hosts at molecular level has been revolutionized with an abundance of new research. This new volume, Advances in Microbial Biotechnology: Current Trends and Future Prospect, focuses on the application of microorganisms for several purposes: for plant protection and improvement, for environmental remediation purposes, and for the improvement of human health. Various applications of microorganisms are covered broadly and have been appropriately reflected in depth in different chapters. The book is divided into four major sections: applied microbiology in agriculture microbes in the environment microbes in human health microbes in nanotechnology The book provides insight into the diverse microorganisms that have been explored and exploited in the development of various applications for agricultural improvements. The book also looks at the application of microbes for the removal of pollutants and the recovery of metals and oils. Also discussed is the detection and exploitation of microorganisms in the diagnosis of human diseases, providing possible holistic approaches to health. This new volume will provide a wealth of information on new research on the application of microbial biotechnology today.

New and Future Developments in Microbial Biotechnology and Bioengineering

New and Future Developments in Microbial Biotechnology and Bioengineering
Trends of Microbial Biotechnology for Sustainable Agriculture and Biomedicine Systems: Perspectives for Human Health

by Ali Asghar Rastegari,Ajar Nath Yadav,Neelam Yadav

  • Publisher : Elsevier
  • Release : 2020-05-15
  • Pages : 312
  • ISBN : 0128205296
  • Language : En, Es, Fr & De
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New and Future Developments in Microbial Biotechnology and Bioengineering: Trends of Microbial Biotechnology for Sustainable Agriculture and Biomedicine Systems: Perspectives for Human Health discusses how microbial biotechnology helps us understand new strategies to reduce pathogens and drug resistance through microbial biotechnology. The most commonly used probiotic bacteria are Lactobacillus and Bifidobacterium. Therefore, the probiotic strains exhibit powerful anti-inflammatory, antiallergic and other important properties. This new book provides an indispensable reference source for engineers/bioengineers, biochemists, biotechnologists, microbiologists, pharmacologists, and researchers who want to know about the unique properties of this microbe and explore its sustainable biomedicine future applications. Introduces the principles of microbial biotechnology and its application for sustainable biomedicine system Explores various microbes and their beneficial application for biofortification of crops for micronutrients Explains the potentials and significance of probiotics, prebiotics and synbiotics in health and disease Includes current applications of beneficial microbes as Functional Food Products of Pharmaceutical Importance

Current Trends in Microbial Biotechnology for Sustainable Agriculture

Current Trends in Microbial Biotechnology for Sustainable Agriculture
A Book

by Ajar Nath Yadav

  • Publisher : Springer Nature
  • Release : 2021
  • Pages : 329
  • ISBN : 9811569495
  • Language : En, Es, Fr & De
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