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Separation Science and Proteomics

Separation Science and Proteomics
Current Trends and New Approaches

by Alexandre Stoyanov

  • Publisher : Academic Press
  • Release : 2017-06-01
  • Pages : 250
  • ISBN : 9780128032619
  • Language : En, Es, Fr & De
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Separation Science and Proteomics: Current Trends and New Approaches provides readers with the most advanced theories and methodologies on the most important separation methods contributing to current proteomics. Intended to be a practical reference that meets the needs of everyday practitioners, it also serves as a go-to guide for scientists focused on the theory of separation science, new methods development, and new equipment design. Future progress in proteomics is connected to the introduction of new and more powerful techniques and the perfection of techniques proposed over the past several decades, and an in-depth understanding of physical mechanisms for a particular separation method or methods combination is crucial for scientists who need to bridge the gap between traditional and contemporary methods. Authored by a chemist who has more than 20 years of experience in separation science and proteomics, this book offers readers insights and solutions to the separation mechanism of a full range of related techniques and complex problems, including the concentration distribution of analyzed protein during IEF separation in natural or IPG gradients or detailed mechanisms of electrophoresis in thermal gradients. Covers theoretical aspects, such as resolving power limitations as flow rate and electric current density reach technical limits Provides a critical review of newly proposed separation methods and approaches, including a range of equilibrium gradient methods Addresses the physical aspects important for optimization of the separation process, including acid–base equilibria, dissociation schemes, and chemistry of non-aqueous solutions Analyzes biophysical methods of protein structure analysis and discusses scaling to micro-separations and possible applications of nanotechnologies

Handbook of Isoelectric Focusing and Proteomics

Handbook of Isoelectric Focusing and Proteomics
A Book

by David Garfin,Satinder Ahuja

  • Publisher : Academic Press
  • Release : 2005-05-23
  • Pages : 334
  • ISBN : 9780120887521
  • Language : En, Es, Fr & De
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Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Polymer Monoliths Photopatterned Within Microfluidic Devices for Proteomics and Separation Science

Polymer Monoliths Photopatterned Within Microfluidic Devices for Proteomics and Separation Science
A Book

by Mei He

  • Publisher : Unknown Publisher
  • Release : 2008
  • Pages : 322
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Gel-free Proteomics

Gel-free Proteomics
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2009
  • Pages : 137
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Introducing Proteomics

Introducing Proteomics
From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

by Josip Lovric

  • Publisher : John Wiley & Sons
  • Release : 2011-06-17
  • Pages : 296
  • ISBN : 1119957192
  • Language : En, Es, Fr & De
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Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric

Separation Methods In Proteomics

Separation Methods In Proteomics
A Book

by Gary B. Smejkal,Alexander Lazarev

  • Publisher : CRC Press
  • Release : 2005-12-12
  • Pages : 536
  • ISBN : 9780824726997
  • Language : En, Es, Fr & De
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Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Analytical Separation Science

Analytical Separation Science
A Book

by Jared Anderson,Alain Berthod,Veronica Pino,Apryll M. Stalcup

  • Publisher : John Wiley & Sons
  • Release : 2016-02-08
  • Pages : 2220
  • ISBN : 3527333746
  • Language : En, Es, Fr & De
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Leading the way for analytical chemists developing new techniques. Introductory Price Available! Order your print copy before 30th April 2016 and save! £650 / $1,075 / €799 List price thereafter: £735 / $1,210 / €899 This new comprehensive 5 volume set on separation science provides a much needed research-level text for both academic users and researchers who are working with and developing the most current methods, as well as serving as a valuable resource for graduate and post-graduate students. Comprising of five topical volumes it provides a comprehensive overview of the subject, highlighting aspects that will drive research in this field in the years to come. Volume 1: Liquid Chromatography Volume 2: Special Liquid Chromatography Modes and Capillary Electromigration Techniques Volume 3: Gas, Supercritical and Chiral Chromatography Volume 4: Chromatographic and Related Techniques Volume 5: Sample Treatment, Method Validation, and Applications Key Features: - Comprises over 2,100 pages in 5 volumes – available in print and online - Edited by an international editorial team which has both prominent and experienced senior researchers as well as young and dynamic rising stars - Individual chapters are labeled as either introductory or advanced, in order to guide readers in finding the content at the appropriate level - Fully indexed with cross referencing within and between all 5 volumes

Separation Methods In Proteomics

Separation Methods In Proteomics
A Book

by Gary B. Smejkal,Alexander Lazarev

  • Publisher : CRC Press
  • Release : 2005-12-12
  • Pages : 536
  • ISBN : 1420028057
  • Language : En, Es, Fr & De
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Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Introduction to Proteomics

Introduction to Proteomics
Tools for the New Biology

by Daniel Liebler

  • Publisher : Springer Science & Business Media
  • Release : 2001-12-04
  • Pages : 198
  • ISBN : 9780896039919
  • Language : En, Es, Fr & De
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Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Proteomic and Metabolomic Approaches to Biomarker Discovery

Proteomic and Metabolomic Approaches to Biomarker Discovery
A Book

by Haleem J. Issaq,Timothy D. Veenstra

  • Publisher : Academic Press
  • Release : 2019-10-24
  • Pages : 504
  • ISBN : 0128197889
  • Language : En, Es, Fr & De
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Proteomic and Metabolomic Approaches to Biomarker Discovery, Second Edition covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods and presents a standard operating procedure for sample selection, preparation and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. This second edition has been fully updated and revised to address recent advances in MS and NMR instrumentation, high-field NMR, proteomics and metabolomics for biomarker validation, clinical assays of biomarkers and clinical MS and NMR, identifying microRNAs and autoantibodies as biomarkers, MRM-MS assay development, top-down MS, glycosylation-based serum biomarkers, cell surface proteins in biomarker discovery, lipodomics for cancer biomarker discovery, and strategies to design studies to identify predictive biomarkers in cancer research. Addresses the full range of proteomic and metabolomic methods and technologies used for biomarker discovery and validation Covers all steps involved in biomarker discovery, from study design to study execution Serves as a vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals and graduate students

Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis
A Book

by Fangjun Wang

  • Publisher : Springer Science & Business Media
  • Release : 2013-12-02
  • Pages : 94
  • ISBN : 3642420087
  • Language : En, Es, Fr & De
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In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

Clinical Proteomics

Clinical Proteomics
From Diagnosis to Therapy

by Jennifer E. Van Eyk,Michael J. Dunn

  • Publisher : John Wiley & Sons
  • Release : 2008-09-08
  • Pages : 694
  • ISBN : 3527622160
  • Language : En, Es, Fr & De
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Unparalleled in its scope and depth, this book brings together proteomic approaches in diagnosis and treatment from all clinical fields, including clinical toxicology. The result is a new discipline in molecular medicine that will revolutionize the treatment and prevention of cancer, stroke and other severe diseases. Following an overview of clinical proteomics, the authors look at the technologies available, before moving on to cancer, cardiopulmonary disease, diabetes and stroke. A whole section is devoted to toxicity and the work is rounded off with a discussion of the future of clinical proteomics.

Genome and Proteome in Oncology

Genome and Proteome in Oncology
A Book

by Fotini Tzortzatou Stathopoulou

  • Publisher : Nova Publishers
  • Release : 2005
  • Pages : 360
  • ISBN : 9781594542855
  • Language : En, Es, Fr & De
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The nature of cancer disease, its probable causes, and the molecular and cellular mechanisms through which malignant tumours develop, have only recently begun to be understood in any appreciable detail. Cancer is fundamentally a disease of the genome, arising from dynamic changes occurring within DNA during the lifetime of the cell e.g. deletions, amplifications, point mutations, translocations that can occur in any cell and that may interact in a variety of cellular pathways. The imbalance in the interplay between genetic and environmental factors can initiate malignancy. The determination of the human genome sequence is acclaimed as one of the great achievements made possible by the rapid progress in the available molecular biology tools achieved during the last few years. The sequence of the human genome promises to unveil invaluable information useful for the development of novel approaches in the diagnosis and treatment of cancer disease. This book includes part of the work of international experts on the most up-to-date developments of various aspects of research on genome and proteome in oncology. the number of genes but in how gene parts are used to build different products in a process of the mRNA transcript called alternative splicing.

Proteome Characterization and Proteomics

Proteome Characterization and Proteomics
A Book

by Timothy D. Veenstra,Richard D. Smith

  • Publisher : Academic Press
  • Release : 2003-10-01
  • Pages : 413
  • ISBN : 9780080569154
  • Language : En, Es, Fr & De
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The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies. Discusses proteomics in the postgenomic age Includes various strategies for quantitative proteomics Covers the role of MS in structural functional proteomics and proteomics in drug discovery and bioinformatics

Monolithic Silicas in Separation Science

Monolithic Silicas in Separation Science
Concepts, Syntheses, Characterization, Modeling and Applications

by Klaus K. Unger,Nobuo Tanaka,Egidijus Machtejevas

  • Publisher : John Wiley & Sons
  • Release : 2010-11-29
  • Pages : 362
  • ISBN : 352763326X
  • Language : En, Es, Fr & De
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Edited by the experts and pioneers in the field, this is the first monograph to cover the topic, containing the must-have information hitherto only scattered among journals. Clearly divided into sections on preparation, characterization and modeling, and applications, this is essential reading for chemists, chromatographers, analytical chemists, biochemists and biologists.

Proteomics in Food Science

Proteomics in Food Science
From Farm to Fork

by Michelle Lisa Colgrave

  • Publisher : Academic Press
  • Release : 2017-04-03
  • Pages : 538
  • ISBN : 0128040572
  • Language : En, Es, Fr & De
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Proteomics in Food Science: From Farm to Fork is a solid reference providing concepts and practical applications of proteomics for those in various disciplines of food science. The book covers a range of methods for elucidating the identity or composition of specific proteins in foods or cells related to food science, from spoilage organisms, to edible components. A variety of analytical platforms are described, ranging from the usage of simple electrophoresis, to more sophisticated mass spectrometry and bio-informatic platforms. The book is designed for food scientists, technologists, food industry workers, microbiologists, and public health workers, and can also be a valuable reference book for students. Includes a variety of analytical platforms, ranging from simple electrophoresis to more sophisticated mass spectrometry and bio-informatic platforms Presents analytical techniques for each food domain, including beverages, meats, dairy and eggs, fruit, fish/seafood, cereals, nuts, and grains that range from sample collection, proportion, and storage analysis Provides applications of proteomics in hot topics area of food safety, including food spoilage, pathogenic organisms, and allergens Covers major pathogens of concern e.g., Salmonella and applications to animal husbandry

Genomics, Proteomics and Vaccines

Genomics, Proteomics and Vaccines
A Book

by Guido Grandi

  • Publisher : John Wiley & Sons
  • Release : 2004-08-13
  • Pages : 336
  • ISBN : 0470093927
  • Language : En, Es, Fr & De
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While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens. This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions

Issues in Chemical, Biological, and Medical Engineering: 2013 Edition

Issues in Chemical, Biological, and Medical Engineering: 2013 Edition
A Book

by Anonim

  • Publisher : ScholarlyEditions
  • Release : 2013-05-01
  • Pages : 660
  • ISBN : 1490108920
  • Language : En, Es, Fr & De
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Issues in Chemical, Biological, and Medical Engineering: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Biosystems Engineering. The editors have built Issues in Chemical, Biological, and Medical Engineering: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biosystems Engineering in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Chemical, Biological, and Medical Engineering: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Proteomics Today

Proteomics Today
Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology

by Mahmoud H. Hamdan,Pier G. Righetti

  • Publisher : John Wiley & Sons
  • Release : 2005-05-13
  • Pages : 448
  • ISBN : 0471709107
  • Language : En, Es, Fr & De
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The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome * Explains the hows and whys of using mass spectrometry inproteomic analysis * Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting

Proteomics for Biological Discovery

Proteomics for Biological Discovery
A Book

by Timothy D. Veenstra,John R. Yates

  • Publisher : John Wiley & Sons
  • Release : 2006-06-12
  • Pages : 320
  • ISBN : 0470007737
  • Language : En, Es, Fr & De
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Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.