Download Stem Cell Bioprocessing Ebook PDF

Stem Cell Bioprocessing

Stem Cell Bioprocessing
For Cellular Therapy, Diagnostics and Drug Development

by Tiago G Fernandes,Maria Margarida Diogo,Joaquim M. S. Cabral

  • Publisher : Elsevier
  • Release : 2013-11-15
  • Pages : 236
  • ISBN : 1908818301
  • Language : En, Es, Fr & De
GET BOOK

Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches Envisages the use of stem cells in regenerative medicine and drug screening applications Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies

Stem Cell Bioprocessing and Manufacturing

Stem Cell Bioprocessing and Manufacturing
A Book

by Joaquim M. S. Cabral,Cláudia Lobato da Silva,Maria Margarida Diogo

  • Publisher : MDPI
  • Release : 2021-01-20
  • Pages : 170
  • ISBN : 3039430386
  • Language : En, Es, Fr & De
GET BOOK

The next healthcare revolution will apply regenerative medicines using human cells and tissues. The aim of the regenerative medicine approach is to create biological therapies or substitutes in vitro for the replacement or restoration of tissue function in vivo lost through failure or disease. However, whilst science has revealed the potential, and early products have shown the power of such therapies, there is an immediate and long-term need for expertise with the necessary skills to face the engineering and life science challenges before the predicted benefits in human healthcare can be realized. Specifically, there is a need for the development of bioprocess technology for the successful transfer of laboratory-based practice of stem cell and tissue culture to the clinic as therapeutics through the application of engineering principles and practices. This Special Issue of Bioengineering on Stem Cell Bioprocessing and Manufacturing addresses the central role in defining the engineering sciences of cell-based therapies, by bringing together contributions from worldwide experts on stem cell biology and engineering, bioreactor design and bioprocess development, scale-up, and manufacturing of stem cell-based therapies.

Bioprocessing for Cell-Based Therapies

Bioprocessing for Cell-Based Therapies
A Book

by Che J. Connon

  • Publisher : John Wiley & Sons
  • Release : 2017-02-06
  • Pages : 272
  • ISBN : 1118743415
  • Language : En, Es, Fr & De
GET BOOK

With contributions from leading, international academics and industrial practitioners, Bioprocessing for Cell-Based Therapies explores the very latest techniques and guidelines in bioprocess production to meet safety, regulatory and ethical requirements, for the production of therapeutic cells, including stem cells. An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout In depth discussion of the application of cell therapy including methods used in the delivery of cells to the patient Includes contributions from experts in both academia and industry, combining a practical approach with cutting edge research The only handbook currently available to provide a state of the art guide to Bioprocessing covering the complete range of cell-based therapies, from experts in academia and industry

Stem Cell Bioprocessing and Manufacturing

Stem Cell Bioprocessing and Manufacturing
A Book

by Joaquim M. S. Cabral,Cláudia Lobato da Silva,Maria Margarida Diogo

  • Publisher : Unknown Publisher
  • Release : 2020
  • Pages : 170
  • ISBN : 9783039430390
  • Language : En, Es, Fr & De
GET BOOK

The next healthcare revolution will apply regenerative medicines using human cells and tissues. The aim of the regenerative medicine approach is to create biological therapies or substitutes in vitro for the replacement or restoration of tissue function in vivo lost through failure or disease. However, whilst science has revealed the potential, and early products have shown the power of such therapies, there is an immediate and long-term need for expertise with the necessary skills to face the engineering and life science challenges before the predicted benefits in human healthcare can be realized. Specifically, there is a need for the development of bioprocess technology for the successful transfer of laboratory-based practice of stem cell and tissue culture to the clinic as therapeutics through the application of engineering principles and practices. This Special Issue of Bioengineering on Stem Cell Bioprocessing and Manufacturing addresses the central role in defining the engineering sciences of cell-based therapies, by bringing together contributions from worldwide experts on stem cell biology and engineering, bioreactor design and bioprocess development, scale-up, and manufacturing of stem cell-based therapies.

Stem Cell Bioprocessing

Stem Cell Bioprocessing
The Bioengineering of Lung Epithelium in 3D from Embryonic Stem Cells

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2009
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Stem Cell Bioprocessing

Stem Cell Bioprocessing
Bioreactor Design and Characterisation by Computational Fluid Dynamics and the Differentiation of Murine Embryonic Stem Cells Into the Alveolar Progenitor Cells in Sparged Bioreactors

by Idtisak Paopo

  • Publisher : Unknown Publisher
  • Release : 2013
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Stem Cell Manufacturing

Stem Cell Manufacturing
A Book

by Joaquim M. S. Cabral,Claudia Lobato da Silva,Lucas G. Chase,Maria Margarida Diogo

  • Publisher : Elsevier
  • Release : 2016-07-24
  • Pages : 340
  • ISBN : 0444632662
  • Language : En, Es, Fr & De
GET BOOK

Stem Cell Manufacturing discusses the required technologies that enable the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic environment as therapeutics, while concurrently achieving control, reproducibility, automation, validation, and safety of the process and the product. The advent of stem cell research unveiled the therapeutic potential of stem cells and their derivatives and increased the awareness of the public and scientific community for the topic. The successful manufacturing of stem cells and their derivatives is expected to have a positive impact in the society since it will contribute to widen the offer of therapeutic solutions to the patients. Fully defined cellular products can be used to restore the structure and function of damaged tissues and organs and to develop stem cell-based cellular therapies for the treatment of cancer and hematological disorders, autoimmune and other inflammatory diseases and genetic disorders. Presents the first ‘Flowchart‘ of stem cell manufacturing enabling easy understanding of the various processes in a sequential and coherent manner Covers all bioprocess technologies required for the transfer of the bench findings to the clinic including the process components: cell signals, bioreactors, modeling, automation, safety, etc. Presents comprehensive coverage of a true multidisciplinary topic by bringing together specialists in their particular area Provides the basics of the processes and identifies the issues to be resolved for large scale cell culture by the bioengineer Addresses the critical need in bioprocessing for the successful delivery of stem cell technology to the market place by involving professional engineers in sections of the book

Bioreactors for Stem Cell Expansion and Differentiation

Bioreactors for Stem Cell Expansion and Differentiation
A Book

by Joaquim M.S. Cabral,Claudia Lobato da Silva

  • Publisher : CRC Press
  • Release : 2018-09-03
  • Pages : 334
  • ISBN : 0429841817
  • Language : En, Es, Fr & De
GET BOOK

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Improving Three-dimensional (3D) Embryonic Stem Cell Bioprocess Design

Improving Three-dimensional (3D) Embryonic Stem Cell Bioprocess Design
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2012
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Centrifugal recovery of embryonic stem cells for regenerative medicine bioprocessing

Centrifugal recovery of embryonic stem cells for regenerative medicine bioprocessing
A Book

by Ju Wei Wong

  • Publisher : Unknown Publisher
  • Release : 2008
  • Pages : 308
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

In order to realise the potential of embryonic stem (ES) cells as a regenerative medicine, it is crucial that economical, robust and scalable bioprocesses be established. Because bioprocesses irrevocably define the safety and efficacy of any biologically derived product, an understanding of the the impact of the engineering environment on ES cells is sought. This thesis uses murine ES cells as a mimic for ES cell types that will be used in cell based regenerative medicine applications to examine the bioprocessing impact of centrifugal recovery cells. A micro scale-down approach was used to examine the effects of centrifugal force, centrifugation time and process temperature on both the yield and biological characteristics of cells subjected to batch centrifugation. When subjected to centrifugation, mES cell loss and cell damage does not appear to occur during the settling or cell pelleting. In general, 5-25% of cells are lost during pellet resuspension to recover the centrifuge cells. The level of cell loss is determined by a combination of centrifugal force, centrifugation time and process temperature. The extent of damage of the remaining cells (i.e. cells not lost during resuspension) is minimised at lower processing temperatures. It is hypothesised that at low processing temperatures, cell loss is minimised due to weak cell-to-cell contact and are thus less susceptible to damage caused by the shear environment generated to disperse the collected cell pellet. The concept of Windows of operations was also applied to evaluate an optimal set of centrifuge operating conditions that results in minimal cell loss and cell damage. The process visualisation tool indicates that operating the centrifuge at 5-9 mins x 300-500 g will result in maximum cell recovery at 4, 21 and 37oC process temperatures. The influence of centrifugation on the biological characteristics of mES cells revealed changes in proliferative capacity, pluripotency and differentiation status when exposed to varying levels of centrifugal force. mES cells exposed to increasing levels of centrifugal force up to 2,000 g progressively lost pluripotency. The pluripotency potential of cells exposed to 3,000 g of centrifugal force was not significantly different from un-centrifuged mES cells. Differentiating mES cells exposed to increasing levels of centrifugal force exhibited increased cell proliferation and a possibility of early induction of endoderm and mesoderm differentiation. Although limited in some areas, the results strongly suggest that restricting exposure to no more than low levels of centrifugal force is necessary to safeguard the stability of the desired mES cell characteristics. Overall, the insight gained from the work accomplished serves to create and establish an awareness of the challenges faced within the arena of whole cell bioprocessing for regenerative medicines.

The Bioprocessing of Stem Cells for Use in Regenerative Medicine

The Bioprocessing of Stem Cells for Use in Regenerative Medicine
A Book

by Pui Kei Carol Chu

  • Publisher : Unknown Publisher
  • Release : 2009
  • Pages : 564
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Real-time Online in Situ Monitoring and Statistical Design Strategies for Haematopoietic Stem Cell Bioprocessing

Real-time Online in Situ Monitoring and Statistical Design Strategies for Haematopoietic Stem Cell Bioprocessing
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2009
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Stem Cell Engineering

Stem Cell Engineering
A WTEC Global Assessment

by Robert M. Nerem,Jeanne Loring,Todd C. McDevitt,Sean P. Palecek,David V. Schaffer,Peter W. Zandstra

  • Publisher : Springer
  • Release : 2014-06-12
  • Pages : 308
  • ISBN : 3319050745
  • Language : En, Es, Fr & De
GET BOOK

This book describes a global assessment of stem cell engineering research, achieved through site visits by a panel of experts to leading institutes, followed by dedicated workshops. The assessment made clear that engineers and the engineering approach with its quantitative, system-based thinking can contribute much to the progress of stem cell research and development. The increased need for complex computational models and new, innovative technologies, such as high-throughput screening techniques, organ-on-a-chip models and in vitro tumor models require an increasing involvement of engineers and physical scientists. Additionally, this book will show that although the US is still in a leadership position in stem cell engineering, Asian countries such as Japan, China and Korea, as well as European countries like the UK, Germany, Sweden and the Netherlands are rapidly expanding their investments in the field. Strategic partnerships between countries could lead to major advances of the field and scalable expansion and differentiation of stem cells. This study was funded by the National Science Foundation (NSF), the National Institutes of Health (NIH) and the National Institute of Standards and Technology (NIST).

Stem Cells and Revascularization Therapies

Stem Cells and Revascularization Therapies
A Book

by Hyunjoon Kong,Andrew J. Putnam,Lawrence B. Schook

  • Publisher : CRC Press
  • Release : 2011-12-13
  • Pages : 342
  • ISBN : 1439803234
  • Language : En, Es, Fr & De
GET BOOK

In the last few decades, significant advancements in the biology and engineering of stem cells have enabled progress in their clinical application to revascularization therapies. Some strategies involve the mobilization of endogenous stem cell populations, and others employ cell transplantation. However, both techniques have benefited from multidisciplinary efforts to create biomaterials and other biomedical tools that can improve and control the fate of stem cells, and advance our understanding of them. Stem Cells and Revascularization Therapies focuses on the fundamentals and applied studies in stem cell biology, and provides perspectives associated with the development of revascularization strategies. To help readers understand the multidisciplinary issues associated with this topic, this book has been divided into four sections: Section 1: Explores how to define, isolate, and characterize various stem and progenitor cell populations for neovascularization Section 2: Summarizes some especially useful model systems and approaches used to regulate angiogenesis, vasculogenesis, and arteriogenesis, and explores their impact on formation of functional vessels in vivo Section 3: Focuses on stem cell homing to sites of injury and inflammation, as well as strategies to exploit this mobilization phenomenon Section 4: Covers stem cell transplantation topics, including recreating features of endogenous stem cell niches to maintain the multipotency of transplanted cells and combinatorial delivery of cells and molecular factors Intended to inspire new contributions to improve the therapeutic efficacy, Stem Cells and Revascularization Therapies outlines emergent findings and challenges regarding the use of stem cells in revascularization therapies. Overcoming the significant hurdles to our understanding of stem cell biology will enhance their utility in promoting new blood vessel formation.

A Novel Design of 3D-bioprocess for Embryonic Stem Cell Expansion and Differentiation: in Vitro Skeletal Lineage Tissue Generation

A Novel Design of 3D-bioprocess for Embryonic Stem Cell Expansion and Differentiation: in Vitro Skeletal Lineage Tissue Generation
A Book

by Jae Min Cha

  • Publisher : Unknown Publisher
  • Release : 2010
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Engineering Strategies for Regenerative Medicine

Engineering Strategies for Regenerative Medicine
A Book

by Tiago G Fernandes,Maria Margarida Diogo,Joaquim M. S. Cabral

  • Publisher : Academic Press
  • Release : 2019-11-14
  • Pages : 284
  • ISBN : 0128166703
  • Language : En, Es, Fr & De
GET BOOK

Engineering Strategies for Regenerative Medicine considers how engineering strategies can be applied to accelerate advances in regenerative medicine. The book provides relevant and up-to-date content on key topics, including the interdisciplinary integration of different aspects of stem cell biology and technology, diverse technologies, and their applications. By providing massive amounts of data on each individual, recent scientific advances are rapidly accelerating medicine. Cellular, molecular and genetic parameters from biological samples combined with clinical information can now provide valuable data to scientists, clinicians and ultimately patients, leading to the development of precision medicine. Equally noteworthy are the contributions of stem cell biology, bioengineering and tissue engineering that unravel the mechanisms of disease, regeneration and development. Considers how engineering strategies can accelerate novel advances in regenerative medicine Takes an interdisciplinary approach, integrating different aspects of research, technology and application Provides up-to-date coverage on this rapidly developing area of medicine Presents insights from an experienced and cross-disciplinary group of researchers and practitioners with close links to industry

Oxygen Monitoring in a Microfluidic Culture Device for Stem Cell Bioprocess Development

Oxygen Monitoring in a Microfluidic Culture Device for Stem Cell Bioprocess Development
A Book

by Anonim

  • Publisher : Unknown Publisher
  • Release : 2014
  • Pages : 266
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
GET BOOK

Development of a Scalable Bioprocess for the Culture of Undifferentiated Murine Embryonic Stem Cells [microform]

Development of a Scalable Bioprocess for the Culture of Undifferentiated Murine Embryonic Stem Cells [microform]
A Book

by Elaine Y. L. (Elaine Yan Ling) Fok

  • Publisher : Library and Archives Canada = Bibliothèque et Archives Canada
  • Release : 2004
  • Pages : 212
  • ISBN : 9780612952089
  • Language : En, Es, Fr & De
GET BOOK

A reliable source of qualified ES cells would enable the development of protocols to test their potential in regenerative medicine models and the design of robust assays to study stem cell differentiation and signaling. Unfortunately, conventional ES cell culture methods are impractical for large-scale cell production under controlled conditions. Two stirred-suspension undifferentiated ES cell culture systems were developed and compared with tissue culture flask and Petri dish controls. Fifteen-day ES cell microcarrier cultures expanded 192 +/- 11.3-fold, with a doubling time of 13.9 +/- 0.7 hrs. (versus 14.8 +/- 1.3 hrs. for tissue flask controls). Cells cultured as aggregates, with agglomeration inhibited by shear, expanded 53.4 +/- 9.6-fold and had a doubling time of 23.5 +/- 5.8 hrs. (versus 20.1 +/- 4.5 hrs. for Petri dish controls). ES cells remained undifferentiated in both systems (i.e., predominantly SSEA-1+, E-cadherin +, Oct-4+). Expected differentiation kinetics and markers were demonstrated upon EB formation. Results suggest that with optimization these systems can support large-scale ES cell production.

Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts

Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts
A Book

by Claire Komives,Weichang Zhou

  • Publisher : John Wiley & Sons
  • Release : 2018-11-27
  • Pages : 288
  • ISBN : 1119378281
  • Language : En, Es, Fr & De
GET BOOK

Written for industrial and academic researchers and development scientists in the life sciences industry, Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts is a guide to the tools, approaches, and useful developments in bioprocessing. This important guide: • Summarizes state-of-the-art bioprocessing methods and reviews applications in life science industries • Includes illustrative case studies that review six milestone bio-products • Discuses a wide selection of host strain types and disruptive bioprocess technologies

Bioprocess Engineering Principles

Bioprocess Engineering Principles
A Book

by Pauline M. Doran

  • Publisher : Academic Press
  • Release : 2012-04-23
  • Pages : 525
  • ISBN : 0080917704
  • Language : En, Es, Fr & De
GET BOOK

This welcome new edition discusses bioprocess engineering from the perspective of biology students. It includes a great deal of new material and has been extensively revised and expanded. These updates strengthen the book and maintain its position as the book of choice for senior undergraduates and graduates seeking to move from biochemistry/microbiology/molecular biology to bioprocess engineering. All chapters thoroughly revised for current developments, with over 200 pgs of new material, including significant new content in: Metabolic Engineering, Sustainable Bioprocessing, Membrane Filtration, Turbulence and Impeller Design, Downstream Processing, Oxygen Transfer Systems Over 150 new problems and worked examples More than 100 new illustrations