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Therapeutic Antibody Engineering

Therapeutic Antibody Engineering
Current and Future Advances Driving the Strongest Growth Area in the Pharmaceutical Industry

by William R Strohl,Lila M Strohl

  • Publisher : Elsevier
  • Release : 2012-10-16
  • Pages : 696
  • ISBN : 1908818093
  • Language : En, Es, Fr & De
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The field of antibody engineering has become a vital and integral part of making new, improved next generation therapeutic monoclonal antibodies, of which there are currently more than 300 in clinical trials across several therapeutic areas. Therapeutic antibody engineering examines all aspects of engineering monoclonal antibodies and analyses the effect that various genetic engineering approaches will have on future candidates. Chapters in the first part of the book provide an introduction to monoclonal antibodies, their discovery and development and the fundamental technologies used in their production. Following chapters cover a number of specific issues relating to different aspects of antibody engineering, including variable chain engineering, targets and mechanisms of action, classes of antibody and the use of antibody fragments, among many other topics. The last part of the book examines development issues, the interaction of human IgGs with non-human systems, and cell line development, before a conclusion looking at future issues affecting the field of therapeutic antibody engineering. Goes beyond the standard engineering issues covered by most books and delves into structure-function relationships Integration of knowledge across all areas of antibody engineering, development, and marketing Discusses how current and future genetic engineering of cell lines will pave the way for much higher productivity

Diagnostic and Therapeutic Antibodies

Diagnostic and Therapeutic Antibodies
A Book

by Andrew J. T. George,Catherine E. Urch

  • Publisher : Springer Science & Business Media
  • Release : 2000
  • Pages : 477
  • ISBN : 1592590764
  • Language : En, Es, Fr & De
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Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer. Antibodies appeared to offer the "magic bullet" that would allow the specific destruction of neoplastic cells. H- ever, many years' effort resulted in very few cases of successful immu- therapy with antibodies. As a result there was a major backlash against antibody therapy, and the field lost a considerable amount of popularity. Fashion, in science as well as in other things, tends to be cyclical. Antibody-based therapy is once again attracting scientists and clinicians. There are several reasons for the renewed optimism; certainly the expe- ence of the last two decades has provided a wealth of information about problems associated with antibody therapy, and possible solutions to these problems. Recombinant antibody engineering has rejuvenated the field, allowing both the modification of antibodies to improve their in vivo pr- erties and the isolation of novel antibody molecules by such techniques as phage display. The results of recent clinical trials have demonstrated unequivocally the benefit of antibody therapy in a number of settings, and, finally, more careful consideration has been taken of the types of disease best treated using this approach.

Therapeutic Antibodies

Therapeutic Antibodies
Methods and Protocols

by Antony S. Dimitrov

  • Publisher : Humana Press
  • Release : 2014-11-25
  • Pages : 585
  • ISBN : 9781627038690
  • Language : En, Es, Fr & De
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Over 2000 years ago in China, antibodies elicited by early forms of vaccination likely played a major role in the protection of the population from infectious agents. Vac- nation has been further developed in Europe and described by Edward Jenner in the late-eighteenth century, then successfully implemented worldwide. The idea to use theactiveingredientinthebloodofvaccinated(orimmunized)animalsorhumansfor the treatment of diseases came a century later. It was made possible by a series of discoveries,suchastherealizationthattheserumfromanimalsimmunizedwithtoxins, for example, diphtheria toxin or viruses, is an effective therapeutic against the disease causedbythesameagentinhumans. Inthe1880s,vonBehringdevelopedanantitoxin (anti-body) that did not kill the bacteria but neutralized the bacterial toxin. The first Nobel Prize in Medicine (1901) was given to him for the discovery of the serum therapy. Acenturylater,22monoclonalantibodies(mAbs)areapprovedbytheUnited States Food and Drug Administration (FDA) for clinical use, and hundreds are in clinicaltrialsforthetreatmentofvariousdiseasesincludingcancers,immunedisorders, and infections. The revenues from the top-five therapeutic antibodies reached $11. 7 billion in 2006, and major pharmaceutical companies raced to acquire antibody biotech companies with a recent example of MedImmune, Inc. , which was acquired for $15. 6 billion by AstraZeneca in 2007. This explosion of research and development in the field of therapeutic antibodies prompted the publication of the MiMB volume Therapeutic Antibodies: Methods and Protocols. The book’s major goal is to present a set of protocols useful for researchers discoveringanddevelopingtherapeuticantibodies. Currentadvancesandfuturetrends in the antibody therapeutics are analyzed in the lead-in review article.

Antibody Engineering

Antibody Engineering
Methods and Protocols

by Benny K. C. Lo

  • Publisher : Humana Press
  • Release : 2010-10-28
  • Pages : 562
  • ISBN : 9781617373527
  • Language : En, Es, Fr & De
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The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Köhler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious—high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host’s immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich’s “magic bullet,” however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15–20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.

Antibody Engineering

Antibody Engineering
A Book

by Roland E. Kontermann,Stefan Dübel

  • Publisher : Springer Science & Business Media
  • Release : 2010-03-10
  • Pages : 788
  • ISBN : 9783642011443
  • Language : En, Es, Fr & De
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Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.

Applications And Engineering Of Monoclonal Antibodies

Applications And Engineering Of Monoclonal Antibodies
A Book

by David J. King

  • Publisher : CRC Press
  • Release : 1998-11-27
  • Pages : 249
  • ISBN : 9780748404223
  • Language : En, Es, Fr & De
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A valuable resource for researchers and workers in the fields of both pharmaceuticals and biotechnology as well as undergraduates in biochemistry, applied biology, biomedical sciences and pharmacy, this book compares established techniques of antibody production with the new. Antibody structure and the implications of antibody engineering are fully discussed, and a case study approach illustrates how antibodies are finding increasing use in the diagnosis and treatment of disease. The volume ends with commercial expression, purification and large-scale manufacture of antibodies and their future potential, particularly as therapeutic agents.

Therapeutic Antibodies

Therapeutic Antibodies
A Book

by Yuti Chernajovsky,Ahuva Nissim

  • Publisher : Springer Science & Business Media
  • Release : 2007-12-11
  • Pages : 380
  • ISBN : 9783540732594
  • Language : En, Es, Fr & De
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This essential work, edited by two researchers at London’s famous Queen Mary’s medical school targets one of the most important areas in medical development today. These days, antibody therapeutics are the treatment of choice for several autoimmune and oncological conditions. They are, indeed, becoming the molecules of choice for further combination therapies and cell engineering. In this timely work, a slew of expert in the field of drug development summarize all the current developments and clinical successes.

Antibody Engineering

Antibody Engineering
Methods and Protocols, Second Edition

by Patrick Chames

  • Publisher : Humana Press
  • Release : 2016-08-23
  • Pages : 734
  • ISBN : 9781493959440
  • Language : En, Es, Fr & De
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More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.

Identification of a Novel Cancer Therapeutic Antibody Against Human Epidermal Growth Factor Receptor 2 (Her2) and Antibody Engineering for Development of Cancer Therapeutics

Identification of a Novel Cancer Therapeutic Antibody Against Human Epidermal Growth Factor Receptor 2 (Her2) and Antibody Engineering for Development of Cancer Therapeutics
A Book

by Chao Chen

  • Publisher : Unknown Publisher
  • Release : 2017-01-26
  • Pages : 129
  • ISBN : 9781361334300
  • Language : En, Es, Fr & De
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Therapeutic Monoclonal Antibodies

Therapeutic Monoclonal Antibodies
From Bench to Clinic

by Zhiqiang An

  • Publisher : John Wiley & Sons
  • Release : 2011-09-20
  • Pages : 896
  • ISBN : 1118210263
  • Language : En, Es, Fr & De
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70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. First book to address the discovery and development of antibody therapeutics in their entirety. Most chapters contain experimental data to illustrate the principles described in them. Authors provide detailed methodologies that readers can take away with them and use in their own laboratories.

Identification of a Novel Cancer Therapeutic Antibody Against Human Epidermal Growth Factor Receptor 2 (Her2) and Antibody Engineering for Development of Cancer Therapeutics

Identification of a Novel Cancer Therapeutic Antibody Against Human Epidermal Growth Factor Receptor 2 (Her2) and Antibody Engineering for Development of Cancer Therapeutics
A Book

by 陳超,University of Hong Kong

  • Publisher : Unknown Publisher
  • Release : 2013
  • Pages : 372
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Engineering Therapeutic Antibody Fragments Targeting the Anthrax Toxin

Engineering Therapeutic Antibody Fragments Targeting the Anthrax Toxin
A Book

by George Robert Mabry

  • Publisher : Unknown Publisher
  • Release : 2005
  • Pages : 129
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Bispecific Antibodies

Bispecific Antibodies
A Book

by Roland E. Kontermann

  • Publisher : Springer Science & Business Media
  • Release : 2011-07-21
  • Pages : 373
  • ISBN : 9783642209109
  • Language : En, Es, Fr & De
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The concept of using bispecific antibodies for cancer therapy by retargeting immune effector cells was developed several years ago. Initial clinical studies were rather disappointing mainly due to low efficacy, severe side effects and the immunogenicity of the bispecific antibodies. The progress in antibody engineering finally led to the generation of new classes of bispecific antibodies lacking these obstacles. In addition, new applications were established, such as pre-targeting strategies in radioimmunotherapy and dual targeting approaches in order to improve binding, selectivity and efficacy. In this book, the different ways of generating bispecific antibodies are described, with emphasis on recombinant formats. The various applications of bispecific antibodies, e.g. in cellular cancer immunotherapy, radioimmunotherapy and pretargeting strategies are covered, and emerging applications such as dual targeting strategies, which involve the simultaneous inhibition of two targets, are addressed.

Introduction to Antibody Engineering

Introduction to Antibody Engineering
A Book

by Florian Rüker,Gordana Wozniak-Knopp

  • Publisher : Springer Nature
  • Release : 2021-01-04
  • Pages : 388
  • ISBN : 3030546306
  • Language : En, Es, Fr & De
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This highly readable textbook serves as a concise and engaging primer to the emerging field of antibody engineering and its various applications. It introduces readers to the basic science and molecular structure of antibodies, and explores how to characterize and engineer them. Readers will find an overview of the latest methods in antibody identification, improvement and biochemical engineering. Furthermore, alternative antibody formats and bispecific antibodies are discussed. The book’s content is based on lectures for the specializations “Protein Engineering” and “Medical Biotechnology” within the Master’s curriculum in “Biotechnology.” The lectures have been held at the University of Natural Resources and Life Sciences, Vienna, in cooperation with the Medical University of Vienna, since 2012 and are continuously adapted to reflect the latest developments in the field. The book addresses Master’s and PhD students in biotechnology, molecular biology and immunology, and all those who are interested in antibody engineering.

Recombinant Antibodies for Cancer Therapy

Recombinant Antibodies for Cancer Therapy
Methods and Protocols

by Martin Welschof,Jürgen Krauss

  • Publisher : Humana Press
  • Release : 2011-10-09
  • Pages : 481
  • ISBN : 9781617372506
  • Language : En, Es, Fr & De
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Since the advent of hybridoma technology more than two decades ago, numerous antibodies have entered the clinical setting as potent therapeutic agents. Their repeated application in humans, however, is limited by the development of human antimouse antibodies (HAMA) in the recipient, leading to allergic re- tions against the foreign murine protein and rapid neutralization. To circumvent these limitations many new antibodies have recently been tailored through recombinant antibody technology. The initial clinical data show encouraging results, thus demonstrating the potential of these new therapeutic agents. The purpose of Recombinant Antibodies for Cancer Therapy is to present a collection of detailed protocols in recombinant antibody technology. It is pri- rily addressed to scientists working on recombinant antibodies as well as cli- cians involved with antibody-based therapies. As with other volumes of this series, we placed the main focus on providing detailed protocols describing procedures step-by-step. Moreover, each protocol supplies a troubleshooting guide containing detailed information on possible problems and hints for pot- tial solutions. Antibody technology is a subject of constant and rapid change. This volume, therefore, does not attempt to cover all possible current experimental approaches in the field. Rather, we present carefully selected protocols, written by competent authors who have successfully verified the particular method described. Given our own professional backgrounds and interest in oncology, we chose to conc- trate chiefly on therapeutic agents for cancer patients.

Cell-line engineering for production of therapeutic antibody glycoforms with increased effector function and characterization of the molecular Fc-Feγ receptor interaction

Cell-line engineering for production of therapeutic antibody glycoforms with increased effector function and characterization of the molecular Fc-Feγ receptor interaction
A Book

by Claudia Ferrara

  • Publisher : Unknown Publisher
  • Release : 2005
  • Pages : 151
  • ISBN : 9876543210XXX
  • Language : En, Es, Fr & De
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Handbook of Therapeutic Antibodies, 3 Volume Set

Handbook of Therapeutic Antibodies, 3 Volume Set
A Book

by Stefan Dübel

  • Publisher : Wiley-Blackwell
  • Release : 2007-03-26
  • Pages : 1228
  • ISBN : 9783527314539
  • Language : En, Es, Fr & De
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In this most comprehensive reference source for the development, production and therapeutic application of antibodies, Volume I contains general chapters presenting established technologies and clinical applications. Volume II provides a look at emerging technologies, new therapeutic concepts, and clinical studies. The third volume features detailed and specific information about each currently approved type of antibody, including the clinical data. A must-have resource for academic researchers, decision makers in industry and healthcare professionals in the clinic.

Antibody Fc Engineering: Towards Better Therapeutics

Antibody Fc Engineering: Towards Better Therapeutics
A Book

by Tianlei Ying,Rui Gong

  • Publisher : Frontiers Media SA
  • Release : 2018-12-21
  • Pages : 129
  • ISBN : 2889456781
  • Language : En, Es, Fr & De
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Antibody Engineering

Antibody Engineering
A Book

by Thomas Böldicke

  • Publisher : BoD – Books on Demand
  • Release : 2018-02-21
  • Pages : 312
  • ISBN : 9535138251
  • Language : En, Es, Fr & De
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Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.

Handbook of Therapeutic Antibodies

Handbook of Therapeutic Antibodies
A Book

by Stefan Dübel,Janice M. Reichert

  • Publisher : John Wiley & Sons
  • Release : 2014-08-04
  • Pages : 2544
  • ISBN : 3527682449
  • Language : En, Es, Fr & De
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Still the most comprehensive reference source on the development, production and therapeutic application of antibodies, this second edition is thoroughly updated and now has 30% more content. Volume 1 covers selection and engineering strategies for new antibodies, while the second volume presents novel therapeutic concepts and antibodies in clinical study, as well as their potential. Volumes 3 and 4 feature detailed and specific information about each antibody approved for therapeutic purposes, including clinical data. This unique handbook concludes with a compendium of marketed monoclonal antibodies and an extensive index. Beyond providing current knowledge, the authors discuss emerging technologies, future developments, and intellectual property issues, such that this handbook meets the needs of academic researchers, decision makers in industry and healthcare professionals in the clinic.